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The Rf-1 gene restores the pollenfertility disturbed by the BT-type malesterile cytoplasm. Nine restrictionfragment length polymorphism (RFLP) markerson chromosome 10, where the Rf-1 geneis located, were converted to PCR-basedmarkers. Genetic analysis was conductedusing the nine markers and a segregatingpopulation consisting of 1042 plants. As aresult, the Rf-1 gene was mappedbetween S12564 Tsp509I...
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