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Genetic diversity, as revealed by eighteenSimple Sequence Repeat (SSR) markers inthirty almond [P. dulcis (Mill.) D.A.Webb], twenty fresh-market peach [Prunus persica (L.), Basch], fifteenprocessing clingstone peach cultivars, andten rootstocks, established the geneticrelatedness among cultivars andcharacterized the variation within andbetween species. One accession each of thewild Prunus species,...
The present study was conducted toinvestigate the genetic inheritance of podcomponents in spring-sown white lupin. Ananalysis of the genetic parameters ofproportion of pod walls and related podcomponents should be helpful to define aselection strategy in the future lupinbreeding programs. Two mating designs wereused, a complete diallel, and a halfdiallel, without reciprocals and withoutselfing. The...
As a prerequisite to improving diseaseresistance and grain quality in Iranianrice cultivars, we determined the geneticrelatedness of popular local cultivars andblast-resistance donor germplasm usingfingerprints derived from simple sequencerepeat (SSR) and plant defense genemarkers. Fifty SSR markers and 28 defensecandidate genes were used to assess thegenetic diversity among popular ricecultivars...
Axillary shoot tips of in vitro grape(Vitis vinifera L. cv. CabernetSauvignon) were successfully cryopreservedby vitrification. Axillary shoot tipswere excised from 4- and 5-month oldplantlets were cultured onsolidified 1/2 MS medium with 1 mg l-1BA at 25 °C. Shoot tips wereprecultured on solidified mediumsupplemented with 0.3 M sucrose for 3 daysand then treated with a mixture of 2 Mglycerol plus...
Flavonoids, in particular the anthocyanins,are responsible for flower colour in manyspecies. The dihydroflavonols represent abranch point in flavonoid biosynthesis,being the intermediates for production ofboth the coloured anthocyanins, through theaction of the enzyme dihydroflavonol4-reductase (DFR), and the colourlessflavonols, produced by flavonol synthase(FLS). In this study the white-flowered,flavonol...
DNA polymorphism between a wild-abortivecytoplasmic male sterile line Zhenshan97Aand its maintainer line Zhenshan97B wasanalyzed using AFLP technique. Among the1953 amplified bands, a stable polymorphicfragment was identified, and designated asA1 in Zhenshan97A and B1 inZhenshan97B. Sequence and homology analysisshowed that A1 contained a more tandemrepeat of 6 base pairs (AGAAAA) thanB1 and the repeat...
To study the origin ofdwarfing genes used in barley breeding inChina, pedigree data of more than 350 dwarfand semi-dwarf cultivars and entriesdeveloped since 1950 were collected. Theresults showed that 68.4% of them werederivatives of just six accessions:`Chibadamai' (CBDM), `Xiaoshanlixiahuang'(XSLXH), `Cangzhouluodamai' (CZLDM),`Aiganqi' (AGQ), `Zhepi 1' and `Yanfu Aizao3' (YFAZ 3). Therefore, these...
Variation in 39 polymorphic RAPD markerswas studied for 18 populations of Solanum anguivi, S. aethiopicum Gilogroup and S. aethiopicum Shum groupin Uganda. UPGMA and PCO analyses of thedata revealed no clear groupingscorresponding to the species and groups. AnAMOVA analysis of the genetic variationshowed that the variation among species andgroups was less than 10%, whereas thevariation within species...
Environmentally and developmentally controlled rosette formation is welldocumented in certain crop species. But this report describes a geneticallyinduced rosetting observed in pomegranate (Punica granatum L.).Rosette genotypes were detected at a frequency of 0.12 in the F2 familiesof cv. Ganesh and a recessive rosette mutant clone of cv. Kabul Yellow. Therosetted seedlings had leaves almost touching...
The wild non-tuberous species Solanumetuberosum is resistant to biotic andabiotic stresses, but is very difficult tocross with cultivated potato. Therefore,interspecific somatic hybrids between adihaploid clone of potato S.tuberosum (2n=2x=24, AA genome) and thediploid species S. etuberosum(2n=2x=24, EE genome) were produced byprotoplast fusion. Among the 7 fertilefusion hybrids analysed by genomic...
In vitro meristem tissue cultures are used for production of virus-free rootstocks of hop (Humulus lupulus L.). Because use of plant tissue cultures is associated with occurrence of somaclonal variability, we assessed somaclonal variability in hop meristem in vitro cultures before and after thermotherapy by different molecular methods (RFLP, RAPD, STS, ISSR and AFLP) and compared it with existing...
Vaccinium elliottii Chapmn., a diploid blueberry in Vacciniumsection Cyanococcus, was crossed with V. arboreum Marsh, a diploid blueberry insection Batodendron. The goal was toproduce hybrids that could be used toincorporate traits from these species intotetraploid southern highbush cultivars. The crosses were made reciprocally usingtwo clones of each species. A recessiveseedling marker gene for...
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