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Common buckwheat is an obligate cross pollinating crop because of its sporophytic self-incompatibility system. Therefore a study to assess the extent of natural outcrossing was undertaken for two years at Morden and Portage la Prairie, Manitoba using the semi-dwarf character, which is due to a homozygous genotype with regard to a single recessive gene, as a marker. The semi-dwarf genotype was grown...
Three isozyme sytems glutamate oxaloacetate transaminase (GOT), superoxide dismutase (SOD), and malic enzyme (ME) have been analysed electrophoretically to study the diversity within and between 33 faba bean (V. faba L. type minor) accessions. For comparison, two cultivars (Vesuvio and Talo) were included in the study. Further objective of this study was to classify populations into genetic similar...
Several races of Fusarium oxysporum Schlechtend.:Fr f. sp. ciceris (Padwick) Matuo and K. Sato cause economic losses from wilting disease of chickpea ( Cicer arietinum L.). While the genetics of resistance to race 1 have been reported, little is known of the genetics of resistance to race 4. We undertook a study to determine the inheritance of resistance and identified random amplified polymorphic...
Comparative analyses of genetic variability of aluminium tolerance response were made in a range of triticale genotypes of two sets, one consisting of six Australian cultivars (or lines), the other consisting of eight South African lines and an Australian check, by following solution culture and screening under controlled growth cabinet conditions. Results showed that Tahara, Tahara ‘S’ and Abacus...
Lentil landraces from South Asia exhibit a low diversity and discordance with landraces from other countries according to a combination of qualitative and quantitative agromorphological characters. They exhibit specific phenological adaptation to the South Asian environment which precludes the direct use of alien germplasm in breeding programs in South Asia. An understanding of the genetic relationships...
Phaseolus vulgaris L. is the most important economic species within the genus Phaseolus, and it is grown in all parts of the world. Genetic improvement by conventional breeding has met considerable success, although production of hybrids between species within the genus has been limited due to sexual incompatibility or other evolutionary lethalities. Recent advances in tissue culture have offered...
Combined effects of sucrose, mannitol and photoperiod on microplant conservation were studied in four potato genotypes belonging to two different groups viz., Tuberosum and Andigena. Minimal growth medium was based on Murashige and Skoog (MS) supplemented with 6 different concentrations of sucrose (30, 40, 50, 60, 70 and 80 gl-1) with 4 different concentrations of mannitol (0, 20, 40 and 60 gl-1)...
Eleven somatic hybrids (2n = 68 to 74) obtained between S. tuberosum ssp. tuberosum cv. Dejima (2n = 48) and ATDH-1 (2n = 24), an anther-culture-derived dihaploid of S. acaule (Yamada et al., 1997), were characterized by nuclear RFLP markers using 49 single-copy DNA probes distributed throughout the potato genome (2 to 6 probes per chromosome). One of the somatic hybrids, DA8-2, had 72 chromosomes...
Immature embryos resulting from the cross V. vexillata × V. unguiculata were cultured on MS medium supplemented with 2,4-D (2 mg/l) and resulted in embryogenic calli. Thirteen hybrid regenerants were obtained via organogenesis by subculturing the calli on MS medium supplemented with BAP (2 mg/l) + adenine sulphate (40 mg/l) + CH (500 mg/l) + cowpea tender pod extract (10%). The interspecific regenerants...
Several morphological and agronomical traits and four independent isozyme loci (PGI1-2, TO1, GOT1 and AcPH) were determined on 20 natural tetraploid populations of Dactylis glomerata subsp. marina and on 7 diploid populations of subsp. izcoi from Galicia (Spain), grown as spaced plants on a sloping, acid sandy loam with summer drought and no fertilization. Diploid izcoi established quicker, had better...
Variation was of root shape in Japanese radish, due to genotypes, soil types and growth stages, were quantitatively evaluated by principal components scores based on elliptic Fourier descriptors. Photographic images of sampled roots on 35mm color reversal films were converted into digital images. After image processing, the contour of each root was expressed as chain-code and then described by 77...
The effect of culture of anthers at 35 °C for one to four days prior to culture at 25 °C in darkness, genotype, anther orientation on callus induction and shoot regeneration in anther culture of flax was investigated. The influence of type and concentrations of cytokinins in the regeneration medium on shoot regeneration was also investigated. The results suggested that culture of anthers at 35 °C...
Genotypes for the glume colour character have been studied in 27 cultivars of common wheat (Triticum aestivum L.) originated from old landraces, and 1 specimen of T. petropavlovskyi Udacz. et Migusch. by means of analysis of the F2 populations. The following tester lines have been used: white-glumed ‘Novosibirskaya 67’ ‘Diamant I’, and ‘Federation’, carrying the Rg1 gene alone; lines RL5405 and near-isogenic...
Forty-one of the major strawberry (Fragaria × ananassa Duch.) cultivars grown in the United States and Canada were examined for RAPD (randomly amplified polymorphic DNA) marker polymorphisms using 10mer primers (>50% GC content). A set of 10 primers produced 15 polymorphic fragments ranging in size between 450 and 1200 bp, which were more than sufficient to distinguish among all tested cultivars...
Inheritance of flower colour and pod colour in cowpea (Vigna unguiculata L. Walp.) has followed a qualitative pattern. Purple flower colour is dominant over white flower colour, whereas black pod colour is partially dominant over white pod colour. A segregation ratio of 3 purple:1 white flowers in F2 generations of two crosses indicated that white flower colour is controlled by a single recessive...
Antifreeze proteins (AFPs) accumulate in the leaves of winter cereals during cold acclimation, where they may inhibit ice recrystallization during freezing and thawing cycles and provide nonspecific disease resistance. In this study, 21 wheat chromosome substitution lines and the parental lines Chinese Spring and Cheyenne wheat were used to determine the heritability of AFPs and the relationship between...
A full diallel analysis is a tool for selection in plant breeding that has been subject to many discussions and controversies regarding its interpretation and merits. The analysis of well-defined transgenes by such an approach permits assessment of the value of diallel analyses. The performance of the Eberhart/Gardner diallel approach is analysed for the β-glucuronidase (GUS) activity of six well-defined,...
The rice (Oryza sativa L.) crop in Arkansas is seeded from late March through early June each year. Farmers need new rice cultivars that not only produce both high rough rice yields and high head rice across this range of seeding dates but do so consistently. Thus, a seeding date study was conducted during 1994 and 1995 at Stuttgart, Arkansas, to study seeding date effects on rough rice yield and...
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