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In order to isolate an elongation factor-1α (EF-1α)gene from cassava, a lambda EMBL3 genomic library, made from a single cassava genotype (MBRA 534, from the CIAT cassava germplasm collection), was screened with a full length EF-1α cDNA clone(blt63) from barley. Six positive clones were isolated from an amplified library and 4866 bp from one clone (MeEF1) were subcloned into pGEM3zf(-) and pGEM5zf(-)...
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