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High seed cost is a major factor limiting the adoption of hybrid rice in South and Southeast Asian countries. A practical way to improve hybrid seed production is to increase the outcrossing rate in rice. Several studies reveal that perennial wild Oryza species have a higher outcrossing rate than annual species. Among various traits that influence outcrossing, pistil characteristics are the most crucial...
The QTL analysis of dead leaf rate (DLR) and dead seedling rate (DSR) during the seedling stage under salt or alkaline stress were conducted, in order to provide the scientific basis for the fine mapping and cloning of QTLs associated with salt or alkaline tolerance, and for the salt or alkaline tolerance of SSR marker assisted rice breeding. The recombinant inbred line (RIL) population F8 including...
To produce rice varieties adaptable to unfavorable environments in Africa, New Rice for Africa (NERICA) varieties have been developed by inter-specific crossings. The basic idea of NERICA was to combine the useful characteristics of the African rice species Oryza glaberrima and an Asian rice species, Oryza sativa . However, the genetic basis of the agronomic characteristics...
Rice sheath blight (ShB), caused by Rhizoctonia solani , leads to severe yield losses in many rice production areas worldwide. Resistance to rice ShB is a typical quantitative trait controlled by quantitative trait loci (QTLs). To identify the QTLs responsible for ShB resistance, phenotypes for ShB resistance have been surveyed in different individuals/lines in various mapping populations...
Cold stress is one of the most severe abiotic adverse factors limiting the growth, productivity, and spatial distribution of rice. We identified a high threshold and low temperature-sensitive mutant ( lts ) in our breeding program. This mutant displays severe cold sensitive phenotypes when the temperature is maintained below 20 °C for 3–5 days. Other abiotic stress responses tend...
The development of two-line hybrid rice has done great contribution to the food security. It is imperative to study the male sterility mechanism of rice photo-thermo sensitive genic male sterile (PTGMS) line which is the core component of two-line hybrid rice. Zhu1S is a rice thermo-sensitive genic male sterile line used frequently as female parent in two-line hybrid seed production. A cytological...
During rice cultivation, brown planthopper (BPH, Nilaparvata lugens Stal.) and white-backed planthopper (WBPH, Sogatella furcifera ) infestations cause decreased yield and rice quality. In this study, we attempted to develop multi-resistance plants and increase the marker-assisted selection (MAS) efficiency. The frequency of callus induction in 95 F 1 doubled...
Seed aging or deterioration in rice (Oryza sativa L.) is a major problem for agronomic production and germplasm preservation. Deciphering the genetic mechanism involved in seed aging and improving seed storability is therefore a vital goal for rice breeding. A set of 85 backcross inbred lines derived from the backcross ‘Sasanishiki’ (japonica cv.)/‘Habataki’ (an indica cv. with strong seed storability)//‘Sasanishiki’...
Cytoplasmic male sterility (CMS) is an important foundation for heterosis utilization and exploited widely to hybrid seed production in rice. However, the traditional experience-driven and labor-cost breeding style left little room for sustainable improvement of hybrid rice. Here, we report a platform for breeding by design of CMS lines based on an SSSL library. A line H121B with rf3...
Diallel crosses have been widely used for analysis of general combining ability (GCA), specific combining ability (SCA) and heterosis. In the present research, 12 lines were selected from 60 inbred rice bred by International Rice Research Institute with extremely-high or -low yielding records according to the previous three consecutive seasons of yield trial experiments, to construct a half...
Bacterial blight disease, caused by Xanthomonas oryzae pv. oryzae ( Xoo ), is one of the most serious diseases in rice producing areas. SA0423 is a broad-range resistance mutant selected from a popular japonica-type variety, TNG67, using sodium azide mutagenesis. Genetic analysis and QTL mapping of SA0423 were performed using the descendants obtained from...
Using a recessive mutant with enhanced salt tolerance at seedling stage obtained from an indica rice cultivar R401 by gamma-ray irradiation, a novel gene controlling salt tolerance in rice was previously mapped to a 406-kb region on chromosome 6. We named the gene Seedling Salt Tolerance ( SST ). In this study, with a large F 2 population derived from a cross...
Grain quality is an important feature of the marketing strategies of the rice industry in both domestic and international markets as quality influences the market price. With the demand for aromatic rice increasing steadily, export of short grain aromatic rices having premium grain quality is a viable option as the cultivation of basmati rices is limited to a specific geographic region. However, these...
Cytoplasmic male sterile (CMS) lines varied at pollen abortive phenotypes (PAP) have been used effectively for production of hybrid seeds in rice. To investigate the relationship between PAP of CMS lines and CMS-specific mitochondrial genes or structures in rice, CMS lines varied at PAP developed on 18 cytoplasmic sources were examined with seven PCR primers specific to three CMS-specific mitochondrial...
Seed germination and seedling establishment are critical phases in rice. In this study, 276 indica accessions were used to investigate the genetic control of seed germination and seedling growth under normal, drought and salt conditions by using the trait of germination percentage, germination index and seedling survival percentage. The significant natural variation of seed germination and seedling...
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