Presents up-to-date developments, methods and techniques in cell culture research involving in vitro systems Describes the applications of cell culture research in human and veterinary medicine, animal cell biotechnology and cell-based bioassays Publishes original papers, reviews and short communications 100% of authors who answered a survey reported that they would definitely publish or probably publish in the journal again Cytotechnology is an international journal covering up-to-date developments, methods and techniques in cell culture research involving in vitro systems, as well as the applications of that research in human and veterinary medicine and animal cell biotechnology. The aim is to centralize information on both the infrastructure of cell technology and the applied use of cell cultures and thus generate a better understanding of the many facets and disciplines needed to develop successful cell culture processes. Topics include the derivation, genetic modification and characterization of cell lines, cell culture techniques, cell culture systems, processes, reactors, scale-up, and industrial production, and the application of cells in a range of investigations, including application in gene therapy and tissue engineering. The journal publishes original papers, reviews and short communications.
Cytotechnology
Description
Identifiers
ISSN | 0920-9069 |
e-ISSN | 1573-0778 |
DOI | 10.1007/10616.1573-0778 |
Publisher
Springer Netherlands
Additional information
Data set: Springer
Articles
Cytotechnology > 2019 > 71 > 6 > 1053-1061
Enterovirus 71 (EV71) infection can cause hand–foot-and-mouth disease (HFMD). Inactivated EV71 vaccine was effective to prevent EV71 derived HFMD. A highly efficient and economical process for producing EV71 is needed. In our study, the epidemic strain of EV71 (EV71-2013ZJHFMD) was obtained and purified. The Vero cells were cultured for production of EV71. The mini-bioreactor vessel (Amprotein Inc...
Cytotechnology > 2019 > 71 > 6 > 1137-1153
In Chinese Hamster Ovary (CHO) cell lines, the establishment of the ideal fed-batch regimen promotes metabolic conditions advantageous for the bioproduction of therapeutic molecules. A tailored, cell line-specific feeding scheme is typically defined during process development (PD) activities, through the incorporation of Design of Experiment (DOE) and late stage cell culture approaches. The feeding...
Cytotechnology > 2019 > 71 > 6 > 1079-1093
Transient gene expression (TGE) bioprocesses have been difficult to scale up in large stirred tank bioreactors with volumes of more than 1.5 L. Low production levels are often observed, but the causes have not been investigated (Gutierrez-Granados et al. in Crit Rev Biotechnol 38:918–940, 2018). Chikungunya Virus-like particle (VLP), expressed by DNA–PEI transient transfection, is a representative...