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In this research, the stabilization effect of cellulase on the decomposition of hydrogen peroxide was investigated for the first time. It was concluded that, regardless of the decomposition mechanism, the cellulase protein could contribute significantly to peroxide stability. This effect stems from the formation of molecular hydrogen bonding between peroxide and cellulase protein or direct sequestering...
Understanding the factors that determine the catalytic efficiency of cellulases is of considerable importance in cellulosic ethanol production, especially at high temperature. The cellulase 12A from the hyperthermophile Thermotoga maritima (TmCel12A) is a possible candidate for accelerating the rate of hydrolysis via temperature elevation up to as high as 95 °C. However, the details of the catalytic...
The Family 7 cellobiohydrolase (Cel7A) from Trichoderma reesei consists of a carbohydrate-binding module (CBM) joined by a linker to a catalytic domain. Cellulose hydrolysis is limited by the accessibility of Cel7A to crystalline substrates, which is perceived to be primarily mediated by the CBM. Here, the binding of CBM to the cellulose Iβ fiber is characterized by combined Brownian dynamics (BD)...
Potato starch processing waste is causing serious environmental problems. This study aimed to convert potato starch processing waste into single-cell protein as high-quality feed using a two-step fermentation process. The mutant strain Aspergillus niger H3 was selected after UV irradiation and ethyl methyl sulfone mutagenesis for more cellulase production. The activities of sodium carboxymethyl cellulase...
Atomic force microscopy in liquid environments (L-AFM) became a state of the art technique in the field of enzymatic cellulose degradation due to its capability of in situ investigations on enzymatic relevant scales. Current investigations are however limited to few substrates like valonia cellulose, cotton linters and processed amorphous cellulose as only these show required flatness and purity....
Cellobiose and glucose are valuable products that can be obtained from enzymatic hydrolysis of cellulose. This study discusses changes in the crystalline form of celluloses to enhance the production of sugars and examines the effect on structural properties during enzymatic hydrolysis. Various crystalline celluloses consisting of group I (cell I, cell IIII, cell IVI) and group II (cell II, cell III...
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