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This study deals with the immobilization of amyloglucosidase within agarose using method of entrapment. Enzyme was produced from Aspergillus fumigatus KIBGE-IB33 and then partially purified using 40% ammonium sulphate saturation. Using 40 gl−1 concentration of agarose and adjusting 3.0 mm size of hydrogels, maximum entrapment yield (78%) was obtained. The kinetic behavior was slightly changed after...
This work reports bioconversion of unsaturated free fatty acids present in soapstock, inherent waste of edible oil manufacture, to the epoxidized fatty acids using Candida rugose lipase in a micro-reactor. Activation energy of the epoxidation reaction was found to be 32.27 kJ mol−1 while the enzyme deactivation was observed at higher temperature than 35 °C with an activation energy of 91.18 kJ mol...
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