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Extracellular recovery of a recombinant, thermostable α-amylase produced by Escherichia coli was increased up to three-fold simply by washing the harvested cells with distilled water. However this phenomenon was confined to this E. coli strain which excretes the same enzyme into the culture fluid. It was demonstrated that the release of α-amylase into the resulting water-wash fraction was not caused...
A competitive enzyme linked immonosorbent assay (ELISA) was developed for quantification of recombinant Bm86 protein (rBm86), antigen of the vaccine GavacTM against the cattle tick Boophilus microplus. Monoclonal antibody (Mab) SSBm1 showed identical recognition to folded and denatured antigen and was used in the competition assay. This ELISA was sensitive enough to detect 60 ng/ml of rBm86. Within-assay...
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