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A filtration flow-through design was used to develop the rapid immunodetection of Escherichia coli. Polyclonal anti-E. coli IgG was conjugated to small, 0.8 μ Blue latex beads. Cells were mixed with conjugated beads in the presence of anti-E. coli monoclonal IgM. The suspension was then filtered through a 5 μ nitrocellulose membrane. The cell-containing complexes were effectively collected on the...
The requirement and supply of reducing power and ATP for the growth-associated synthesis of polyhydroxybutyrate (PHB) and the non-PHB biomass were balanced. By using glycerol, glucose, fructose, octanoate or oleate, the net balances of the PHB synthesizes result in an energy surplus which might contribute to growth. The calculated yields amount up to 90% PHB in cells correlating inversely to the P/O-quotient...
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