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In order to establish human oral epithelial cell lines, secondary cultures of human oral keratinocytes from normal gingival tissues obtained from 20–29-yr-old females were transfected with origin (−) SV40 DNA (pRNS-1) and human c-fos gene (pBK 28) using Transfectam reagent® (Promega, WI). Out of 20 cultures transfected with pRNS-1, only one culture escaped senescence. After the transfection with origin(−)...
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