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GABA-gated Cl channels were studied in the nervous system of the larval tobacco hawk moth, Manduca sexta, using electrophysiology, 36Cl uptake into membrane microsacs and immunocytochemistry. A GABA-induced increase in Cl conductance was recorded from a visually identifiable neurone (fg1) in the desheathed frontal ganglion. The response was insensitive to the vertebrate GABAA receptor antagonist,...
The noncompetitive antagonist of ionotropic γ-aminobutyric acid (GABA) receptors 4′-ethynyl-4-n-propylbicycloorthobenzoate (EBOB) is a useful tool to probe the antagonist-binding site. In the present study, four mutants of the human GABAA receptor β3 subunit were stably expressed in S2 cells and examined for their abilities to bind [3H]EBOB to identify the binding site of EBOB. The homo-oligomeric...
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