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In this study the identification of epigenetic marks of closed and open chromatin states has been performed by immunofluorescence in vibratome-thick sections from poplar stems, followed by quantification of the signal in a representative population of individual nuclei on a confocal microscope. Unlike other methods often used for detection of global DNA methylation levels, the procedure hereby proposed...
From the start of autumn and throughout winter dormancy, starch stored in the stems and buds of woody plants is broken down into oligosaccharides, mainly raffinose family oligosaccharides (RFOs). Given that RFO synthesis protects plants against winter cold, the enzyme galactinol synthase (catalyzing the first step in RFO synthesis) could play an important role in this process. Here, we characterized...
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