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We have examined the organization of the pretectal area in two monotremes (the short beaked echidna—Tachyglossus aculeatus, and the platypus—Ornithorhynchus anatinus) and compared it to that in the Wistar strain rat, using Nissl staining in conjunction with enzyme histochemistry (acetylcholinesterase and NADPH diaphorase) and immunohistochemistry for parvalbumin, calbindin, calretinin and non-phosphorylated...
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