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Abstract. In a search for membrane docking proteins interacting with Zea mays auxin-binding protein (ABP1) the binding of purified ABP1 to maize coleoptile plasma-membrane vesicles was investigated. Concentration-dependent, saturable binding of ABP1 to the membrane vesicles was observed in binding assays using 108106M ABP1. Biotinylated ABP1 was displaced from the membrane binding sites by competition...
Abstract. The NADH-dependent nitrate reductase (NR, EC 1.6.6.1) in roots of hydroponically grown barley seedlings was extracted, desalted and the activity measured in buffer containing either Mg2+ (10mM) or EDTA (5mM). The former gives the actual NR activity (NRact) equivalent to dephospho-NR, whereas the latter gives the maximum NR capacity of the dephospho-form (NRmax). Both values together permit...
Abstract. The conducting elements of phloem in angiosperms are a complex of two cell types, sieve elements and companion cells, that form a single developmental and functional unit. During ontogeny of the sieve element/companion cell complex, specific proteins accumulate forming unique structures within sieve elements. Synthesis of these proteins coincides with vascular development and was studied...
Abstract.Kalancho pinnata (Lam.) Pers. (Crassulaceae), a succulent-leaved crassulacean-acid-metabolism plant, was grown in open-top chambers at ambient and elevated (two times ambient) CO2 concentrations under natural conditions at the Smithsonian Tropical Research Institute, Republic of Panama. Nocturnal increase in titratable acidity and nocturnal carbon gain were linearly related, increased with...
Abstract. To investigate the effect of water stress on carbon metabolism in growing potato tubers (Solanum tuberosum L.), freshly cut and washed discs were incubated in a range of mannitol concentrations corresponding to external water potential between 0 and 1.2 MPa. (i) Incorporation of [14C]glucose into starch was inhibited in water-stressed discs, and labeling of sucrose was increased. High glucose...
Abstract. Five major soluble nuclear proteins associated with cell proliferation were identified in Allium cepa L. root cells. One of them, of 64kDa, was revealed by Western blotting with anti-mammalian nucleolin antibodies. A polyclonal antibody raised against this protein, which we have named NopA64, localised it in the nucleolus as well as in nuclear coiled bodies. Together with NopA64, the antibody...
Abstract. Plant regeneration via somatic embryogenesis was achieved from leaf petioles of Pelargonium sp. `Frensham cultured on Murashige and Skoog medium containing 15 M N6-benzyladenine, and 5 M -naphthaleneacetic acid (NAA). More than 80% of these somatic embryos converted into plants when isolated and cultured on Murashige and Skoog medium supplemented with 15 M NAA. Stable transgenic plants were...
Abstract. Whole-cell patch-clamp techniques were used to measure anion currents through the plasma membrane of protoplasts of mesophyll cells of expanding pea (Pisum sativum L.) leaves. Voltage-induced changes of the currents could be modelled with single exponential activation and deactivation kinetics. The anion currents were activated at negative membrane potentials. The time constant of activation,...
Abstract. Jasmonic acid (JA) is thought to be part of a signal-transduction pathway which dramatically increases de-novo nicotine synthesis in the roots and increases whole-plant (WP) nicotine pools in response to the wounding of the leaves in Nicotiana sylvestrisSpegazzini and Comes (Solanaceae). We report the synthesis of a doubly labeled JA ([1, 2-13C]JA) and use it as an internal standard to quantify...
Abstract. Light-stress-related changes in photosystem I (PS I) were analyzed in photoautotrophic cultured cells of Marchantia polymorpha L. High light treatment (30h; 1300 mol photonsm2s1) reduced the PS I-mediated electron-transport rate by more than 50% and the photochemical efficiency of photosystem II (PS II) by about 35%. In photoinhibited cells, 76% of the PS II centers remained closed in low...
Abstract. A soybean cytosolic glutamine synthetase gene (GS15) was fused with the constitutive 35S cauliflower mosaic virus (CaMV) promoter in order to direct overexpression in Lotus corniculatus L. plants. Following transformation with Agrobacterium rhizogenes, eight independent Lotus transformants were obtained which synthesized additional cytosolic glutamine synthetase (GS) in the shoots. To eliminate...
Abstract. Gibberellins A1 and A3 are the major physiologically active gibberellins (GAs) present in young fruit of pea (Pisum sativum L.). The relative importance of these GAs in controlling fruit growth and their biosynthetic origins were investigated in cv. Alaska. In addition, the non-13-hydroxylated active GAs, GA4 and GA7, were identified for the first time in young seeds harvested 4d after anthesis,...
Abstract. The removal of Ca2+ from the medium by intact vacuoles and microsomes of Chenopodium album was investigated by measuring INDO-1 fluorescence emission at 400 and 480 nm and the response of Ca2+ -selective mini-electrodes. The removal of Ca2+ depended on the presence of MgATP, displaying an apparent KmATP of about 50 M, a KmCa of 400500 nM, and a nucleotide specificity (%) of ATP (100)CTP...
Abstract.The temperature dependence of metabolic rates determines how plant growth rates vary with temperature. This paper shows that equations on physiological relations between respiration rates (i.e. rates of heat loss and CO2 evolution) and growth rates can be used to describe temperature effects on plant growth rate. Incorporating measured values of plant respiratory heat and CO2 rates at a few...
Abstract. Open-flow assays of H2 evolution in Ar:O2 (80:20, v/v) by nodulated roots were performed in situ with soybean [Glycine max (L.) Merr.] and alfalfa [Medicago sativa L.) grown in sand with orthophosphate (Pi) nutrition either limiting (low-P) or non-limiting (control) for plant growth. Nodule growth was more limited than shoot growth by P deficiency. Phosphorus concentration was less affected...
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