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Abstract. An improved protocol for Agrobacterium-mediated transformation of Duncan grapefruit (Citrus paradisi Macf.) epicotyl explants was developed by examining the effects of six different factors on the efficiency of transformation and combining the best treatment for each factor. The preculturing of explants and the composition of the cocultivation medium were the factors that most influenced...
Abstract. Grapefruit (Citrus paradisi Macf. cv Duncan) plants were transformed with several sequences from citrus tristeza closterovirus (CTV) that varied in terms of position in the CTV genome and virus strain origin in an attempt to obtain resistant plants. The sequences included the capsid protein gene from three different strains, a nontranslatable version of the capsid protein gene, the replicase...
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