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A reliable cryopreservation technique was developed for friable embryogenic callus lines of Hevea brasiliensis. The study showed that reducing the CaCl2 concentration of the pre-culture medium from 9 mM to 1 or 0 mM CaCl2 before cryopreservation promoted post-thaw callus growth, 1 mM being the optimum CaCl2 concentration for embryo regeneration. Post-thaw callus proliferation decreased in line with...
In Hevea tree, rubber elongation factor (REF) is a key gene involved in rubber biosynthesis. Since the immaturity period for Hevea is 6 years, identification of molecular marker for latex yield potential will be beneficial for early selection of high yielding clones. The main objective of this research is to study the expression pattern of the REF gene in contrasting latex yield rubber clones (high...
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