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AbstractCells of a suspension culture of Doritaenopsis cv. New Toyohashi were placed in a mixture of 2M glycerol and 0.4M sucrose for 15min at room temperature and then dehydrated with a vitrification solution (PVS2) for 13h on ice and plunged into liquid nitrogen. The highest viability (64% by 2,3,5-triphenyltetrazolium chloride stainability) was obtained when the cells were precultured in liquid...
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