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The N terminus of peptides generated by AspN is restricted to about 40 dipeptide motifs starting with D or E. These motifs are visible upon collision-induced dissociation (CID) as b2 ions, which are often the most abundant low-mass fragment ions. It was observed that b2 ions are accompanied by a set of sequence-specific neutral losses of CO, H2O, NH3, and some other small units. To test the utility...
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