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Polyclonal antisera to β-amanitin were generated in sheep and used to construct a competitive ELISA for measurement of the toxin in human serum and urine. The assay had a detection limit of about 80 pg mL−1, a dynamic range of 80–2,000 pg mL−1, a cross reactivity of 22% with α-amanitin, and no cross reactivities with cyclic peptides from algal sources. Assay responses in buffer, serum, and urine were...
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