Testosterone is the main androgenic hormone produced by Leydig cells, found mainly in testicles/ testis. The aim of this study was to review the latest research on the effects of zinc, magnesium and vitamin D on testosterone levels among male athletes..Research suggests that the optimization of zinc supply may result in enhanced androgen synthesis but only in case of an earlier deficiency of this element. Regarding magnesium, there are indications based on intervention studies that indicate the positive influence of magnesium supplementation (alone or in combination with zinc)on testosterone synthesis. Due to the presence of vitamin D receptors (VDRs) and enzymes metabolizing this vitamin in Leydig cells, the effect of providing cholecalciferol on testosterone synthesis seems probable, but the available studies do not explain the strength of this effect.