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We developed a custom-design video-rate intravital laser-scanning microscopy system for direct in vivo cellular visualization. We visualized the dynamic behavior of flowing T lymphocytes in lymph node and the molecular absorption in small intestine.
Bessel beams have been used in many applications due to their unique optical properties of maintaining their intensity profiles unchanged during propagation. In imaging applications, Bessel beams have been successfully used to provide extended focuses for volumetric imaging and uniformed illumination plane in light-sheet microscopy. Coupled with two-photon excitation, Bessel beams have been successfully...
Surface-plasmon enhanced microscopy has been investigated using blocked random nanodot arrays. J744 cells were imaged on blocked nanodot arrays under total internal reflection. The images were deconvolved based on localized near-field distribution for super-resolution. Experimentally achieved resolution is estimated to be 100-150 nm. Effects of signal-to-noise and registration on the reconstructed...
The rise of various optical clearing methods provides a great potential for imaging deep inside tissues by combining with multiple-labelling and microscopic imaging techniques. However, these methods had certain weaknesses, including toxicity of reagents, tissue deformation, fluorescence quenching, and complexity of implementation. In this study, we developed simple and rapid clearing agents for improving...
A simple tissue optical clearing method was developed based on ClearT, which can clear Neonatal (P2) whole-brain within one day. The optical clearing efficacy of adult mice brain samples is much more than that of ClearT and ClearT2The developed optical clearing method does not affect the EGFP fluorescence.
Soliton self-frequency shift (SSFS) is a highly versatile technology for generating femotosecond pulses with broadband wavelength tunability. Here we review our recent progress in utilizing SSFS for various modalities of multiphoton microscopy, including both fluorescence microscopy and harmonic generation microscopy.
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