The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
The DNA fragments of mpb63 and mpb64 were fused by splicing by overlapping extension(SOE) polymerase chain reaction(PCR), and the fusion gene mpb63–64 was cloned into pMD18-T vector, then we got the recombinant plasmid pMD-63–64. pMD-63–64 and pET28a(+) were digested by BamH□and EcoR□ double enzymes.The purified mpb63–64 fusion gene was subcloned into the expression vector pET28a(+), and the prokaryotic...
Aim: To study the effect of Geraniin on the expression of P53 genes in human hepatoma HepG2 cells. Methods: Cell survival was measured using MTT metabolic viability assay. Apoptosis of HepG2 was investigated by DNA gel electrophore; The level of P53 gene expression was measured by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting analysis after the HepG2 cells were treated...
In order to understand the MMP-2 and TIMP-2 expression of sclera and scleral fibroblasts after posterior sclera reinforcement, three-week-old rabbits were monocularly performed by eyelid suturation randomly to prepare experimental myopia eye. After 60 days, the experimental myopia eyes were treated by posterior scleral reinforcement. After 6 months the posterior pole sclera tissue and scleral fibroblasts...
In this study, a glucose biosensor model was developed. The experiment was performed by connecting commercial amperiometric glucose biosensor with transimpedance amplifier (TIA) readout circuit. By supplying various AC signals and glucose solutions to the biosensor, the resistance and capacitance of the biosensor was found. In conclusion, an amperiometric glucose biosensor model is a parallel combination...
The US4 open reading frame of the Duck enteritis virus (DEV) encodes glycoprotein G, a protein comprising 459 amino acids with a putative molecular mass of 50.58 kDa. The characteristics of this protein were analyzed through bioinformatics softwares and tools. Glycoprotein G sequence analysis indicates that the protein possesses typical characteristics of membrane glycoproteins containing two transmembrane...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.