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Baoding city locates on northwestward of the North China Plain, middle section of the Taihang Mountain which is a typical city on groundwater developing in China. Authors summarize the history of groundwater use and contributions of groundwater, analyze environmental geological issues caused by over-exploitation of urban groundwater which are similar with other water-shortage cities. Main environmental...
This project focuses on the improvement of NDV production technology and innovation of the NDV vaccine preparation process with cell culture technology. Methods: To use primary chick embryo cell culture system in preparation of NDV anti-tumor vaccine. Results:In the new vaccine development process, the focused on maintaining and improving NDV titer while reducing the residual bovine serum. So that...
Pesticides are widely used in China for crop protection. Econometric analysis showed the magnitude of the chronic health effects and health costs to be directly related to pesticide exposure, and accumulative pesticides has led to serious pollution to the environment. This study was conducted on a random sample of 150 rice farming households to collect information on their knowledge, attitude, and...
To investigate the optimum culture conditions of γ-aminobutyric acid (GABA) production by a strain CC-W11 identified as Lactobacillus brevis in submerged fermentation, certain fermentation parameters involving inoculum volume of seeds, incubation temperature, initial pH value, period of cultivation and glutamate concentration in medium were investigated separately. The optimal cultivating conditions...
For the red mud is the main waste of aluminium works which pollutes the water environment. In order to utilization the red mud, the CaO-Fe3O4-Al2O3 flux for hot metal pretreatment is developed. The phase diagrams of the CaO-Fe3O4-Al2O3 system at 1350°C and 1600°C was calculated and analyzed with the FactSage software, and verified by the experimental results. In the phase diagram of the CaO-Fe3O4-Al...
The shallow city river of Baoyang located in China had been eutrophicating with nutrients from domestic sewages for many years. A heavy bloom of Microcystis.aeruginosa in Baoyang River was recorded in 2004 and microcystin-RR (MCRR), the most popular cyanobacterial hepatotoxin microcystins in China, could be detected in the river in the next several years in the filtered waters. To determine the environmental...
Abstract-Acidibility and acidifying degree were important indicators for acidification process in anaerobic digestion. Four factors affecting acidibility and acidifying degree termed temperature, sludge inoculum, mixing speed and substrate concentration were investigated on the basis of an orthogonal test. The order of the four factors affecting the acidification process was temperature > sludge...
In order to obtain a higher glutathione (GSH) production, the original strain of S. cerevisiae BY-14 was mutated with N+ implantation (10 keV, 60×1014 ions/cm2) , and an ethionine-resistant mutant BL-23 was obtained. The mutant showed GSH production increased from 58.79 mg/l to 113.46 mg/l, and content increased from 14.17 mg/g dry cell weight to 20.86 mg/g dry cell weight. The mutant BL-23 is stable...
The pollution would be made during the procession of Soy Protein Concentrate. In the waste of SPC, there are protein due to defferrent alcohol destiny. To recovery them effectively, the paper studied systematically the effect of different alcohol concentrations on the molecular weight of soybean protein concentrate in the extraction which was determined by SDS-PAGE electrophoresis and dissolution...
Maturity of compost is an important practical factor in agricultural and horticulture production. This study was conducted to perform maturity assessment for spent mushroom compost (SMC). Compost maturity were evaluated using standard chemical analyses (pH, EC, CEC, total C and N content, C/N ratio, NH4+-N, NO3--N). Subsequently, potential phytotoxicity from SMC was assessed by germination bioassay...
The paper focuses on the pressure of China's consumption on resources and environment with the economic development. The paper shows that an increase in consumer demand places more pressure on the environment, there are distinct differences of economic and social circumstances from regions to regions; and when looking at the social formation, there is typical dual economic and social structure in...
The feasibility of co-digestion of turf cutting litter and sewage sludge was investigated. The cutting litter were mixed with the sewage sludge at the ratio of 10%, 20%, 30%, 40% and 50%, respectively, based on the dry mass. Under thermophilic anaerobic condition, the gas production rose with the increase of cutting litter ratio. When the cutting litter ratio was 50%, the digestion system produced...
The efficiency of excess sludge disintegration play a major role in protein extraction qualification. Hence, the use of optimal method is likely to promote the study of protein extractive rate in excess sludge. The objective of this study was to develop protein extraction by papain hydrolysis. There are four different factors were correlated to the protein yield during papain hydrolysis. These factors...
Hydrogenase is the key terminal enzyme in electron delivery within hydrogen metabolism in bacteria, which has been attracted many attentions. In the present study, crude enzyme was firstly prepared by cell broken. Then a kind of hydrogenase from Ethanoligenens harbinense YUAN-3 was purified by chromatography methods on Sephadex G-100 and DEAE 52 columns. The enzyme was purified 169-fold with 7.8%...
To obtain the rapid startup of EGSB reactor seeded with anaerobic digestion sludge for the treatment of actual domestic wastewater under ambient temperature, two startup methods i.e. A and B were tried out at 25°C. For method A, reactor A (RA) was fed with brewery wastewater to incubate granular sludge and then treated domestic sewage, for method B, reactor B (RB) was fed with sanitary waste directly...
China is a great agricultural country. Reasonable development and utilization of rural energy is related to Peasant's human production and life and improvement of rural eco-environment. Because lack of energy is serious in recent years. China strengthened study on biogas technology and got rich findings. But there is relatively backward in extended application of biogas technology. Therefore,this...
The pulA gene enconding a thermostable pullulanase of 844 amino acids was cloned by PCR amplification from the genomic DNA of Thermotoga maritima MSB8. We successfully expressed the pulA gene in a protease-deficient strain Bacillus subtilis WB600, and the expressed pullulanase was secreted into the culture broth. Preliminary results showed that the pullulanase exhibited optimal activity at 90°C, and...
Control-effective flux(CEF) analysis is a useful tool in assessing a metabolism. In this work, a model that utilizes existing knowledge of oxygen and redox sensing/regulatory system to assist elementary flux modes (EFMs), has been developed and CEF analysis was performed to analyze the influence of oxygen level on the transcriptional regulation in Klebsiella pneumoniae. It was found that the efficiency...
Deoiled Jatropha curcas seed meal (DJSM), a chief waste material of oil extraction industry, will be generated on a large scale due to the growing interest in using Jatropha curcas seed oil as a feedstock for the production of biodiesel. Up to now, there is very little work on viable approach for the use of DJSM to the best of our knowledge. This article reports our studies on the utilization of this...
Chinese medicinal insects were added into the media to investigate their effects on the mycelial growth and polysaccharide formation by Ganoderma lucidum SCIM 0819. The experiments were carried out in submerged fermentation using shake flasks. The results show that addition of C. molossus at 5.0 g/L lead to significant increase in intracellular polysaccharides (IPS) concentration from 1.48 ± 0.12...
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