The Infona portal uses cookies, i.e. strings of text saved by a browser on the user's device. The portal can access those files and use them to remember the user's data, such as their chosen settings (screen view, interface language, etc.), or their login data. By using the Infona portal the user accepts automatic saving and using this information for portal operation purposes. More information on the subject can be found in the Privacy Policy and Terms of Service. By closing this window the user confirms that they have read the information on cookie usage, and they accept the privacy policy and the way cookies are used by the portal. You can change the cookie settings in your browser.
A variety of extracellular matrix (ECM) scaffolds have been promising in various tissue engineering applications, such as the porcine small intestinal submucosa. Yet no particular scaffolds have been optimal for vocal fold regeneration. The decellularized human umbilical vein (HUV) is a novel allogeneic scaffold that has shown some promise for cardiovascular tissue engineering. This study examines...
Rotator cuff tears are among the most common shoulder injuries that require surgery. High failure rates of biological graft-based repairs underscore the need for functional alternatives. In this study, effects of nanofiber organization on adhesion of cuff fibroblasts (hRCF) and human mesenchymal stem cells (hMSC) are evaluated. It is hypothesized that fiber alignment will regulate cell morphology...
Rotator cuff tears are among the most common shoulder injuries that require surgery. High failure rates of biological graft-based repairs underscore the need for functional alternatives. Specifically, functional grafts must incorporate the gradient of mineralization from tendon to bone in order to be biomimetic. In this study, effects of varying concentrations of mineral content in aligned nanofiber...
The goal of this study is the complete simulation of the structural and the functional components of the extra-cellular matrix using semi-synthetic polysaccharides for tissue engineering applications.
Embryonic stem cells (ESCs) hold promise either as an in vitro model recapitulating early embryonic development or as a renewable source of therapeutically useful cells. However, 2D culture system, which is still generally utilized in ESCs differentiation strategies, has greatly impeded our learning more information about ESCs proliferation/differentiation in situ. Here, a high aspect ratio vessel...
Tissue engineering within three-dimensional scaffolds represents a developing field with the potential to create in vitro livers. Human liver cells, HepG2, respond to cues in their immediate microenvironment by modulating cell functions including cell proliferation, urea synthesis and drug metabolism. The importance of three-dimensional environments draws from the fact that it has been extensively...
ECM is useful in providing growth and support to the cells as well as engineered tissues. There are various techniques for producing artificial matrix constructs to simulate the ECM. These include wet spinning, phase separation, dry spinning, and electrospinning. Electrospinning has advantages over other techniques as it can produce scaffolds which have a high surface area to volume ratio. Electrospinning...
It is critical to fabricate ideal scaffolds for tissue engineered vascular graft. Nanofibers composted with collagen, elastin and polycaprolactone (PCL) were prepared by electrospinning. The obtained composite fibers had an average diameter around 300-500 nm. Mouse smooth muscles cells and endothelial cells cultured on these composite fibrous meshes showed enhanced cell attachment and accelerated...
The objective of this study was to fabricate 3-dimensional (3D) chitosan nanofiber scaffolds using an electrospinning technique and explore its potential for cardiac tissue engineering. Three culture conditions were tested: cardiomyocytes only, cardiomyocytes-fibroblasts cocultures, and cardiomyocyte-endothelial cells cocultures. The cells were seeded on 2-dimensional (2D) chitosan films and 3D chitosan...
The pluripotency of human embryonic stem cells (hESCs) provides the unique opportunity to derive tissue specific cells, an ability of particular interest in regenerative medicine. In the study of bone regeneration, there is interest in directing hESCs to mesenchymal stem cells (MSCs), which under the appropriate conditions gives rise to mineral-producing osteoblasts. To evaluate the efficiency of...
Rotator cuff tears often occur at the tendon-bone insertion site, a fibrocartilaginous interface that is not regenerated during current repairs. Our aim is to regenerate the insertion site through use of extracellular matrix based scaffolds. In this study, we evaluated the response of mineralized and non-mineralized nanofiber scaffolds to cell culture environment without the influence of cell growth...
As more materials and soluble molecules are developed, the ability to screen these materials for applications in tissue engineering becomes increasingly important. A combinatorial library of poly(??-amino ester)s was screened to identify an osteoconductive material based on material properties and cellular interactions. The optimal material, A6, was then implanted into a critical-sized cranial defect...
This work involves the study of the origin and effect of space charges in the electrospinning process. Electrospinning is a process used to make solid, nanofibrous polymer mats. These mats are used in tissue engineering as scaffolds for cells. The origin and effect of space charges in this process has not been well-studied.
The purpose of this study is to develop biodegradable nerve guidance conduits seeded with Schwann cells (SC) using chitosan-based biopolymers for improving nerve regeneration. Chitosan, prepared from alkaline hydrolysis of chitin, is a natural polysaccharide biopolymer, having biocompatibility and biodegradability. In this study, chitosan and chitosan-gelatin membranes were prepared, characterized,...
Spinal cord injury (SCI) affects thousands of Americans each year. The injury results in local cell loss in the spinal cord, interrupting the connections between brain and periphery. Current treatment options for SCI are limited due to the inability of adult neurons to regenerate in the inhibitory environment of the injured central nervous system (CNS). The primary goal of this work is to design a...
Vascularization for adequate supplies of oxygen and nutrients is one of the challenges in tissue engineering. We report the three-dimensional cell printing technology to construct vascular structure in hydrogel scaffold which consist of endothelial cells and collagen scaffold. We constructed multi-layered, inter-connected channels within collagen hydrogel through layer-by-layer approach. Collagen...
Set the date range to filter the displayed results. You can set a starting date, ending date or both. You can enter the dates manually or choose them from the calendar.