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Current delivery systems for vaccines present major logistical problems when large populations need to be vaccinated within a short time period, such as in the case of a pandemic. An efficient and effective method for pandemic vaccination is the driving force of the research performed by our group. The proposed alternative vaccination technique consists of a self-administrable patch made of electrospun...
Rotator cuff tears are among the most common shoulder injuries that require surgery. High failure rates of biological graft-based repairs underscore the need for functional alternatives. In this study, effects of nanofiber organization on adhesion of cuff fibroblasts (hRCF) and human mesenchymal stem cells (hMSC) are evaluated. It is hypothesized that fiber alignment will regulate cell morphology...
This paper reports a new method to induce osteogenic differentiation of human embryonic stem cells (hESCs). Singly dissociated hESCs treated with ROCK inhibitor Y27632 were plated on type I fibrillar collagen (FC) coatings and achieved a high rate of survival. After culturing in osteogenic medium for 10 d, several cell colonies developed on the coatings. Cells forming the colonies showed homogenous,...
It is critical to fabricate ideal scaffolds for tissue engineered vascular graft. Nanofibers composted with collagen, elastin and polycaprolactone (PCL) were prepared by electrospinning. The obtained composite fibers had an average diameter around 300-500 nm. Mouse smooth muscles cells and endothelial cells cultured on these composite fibrous meshes showed enhanced cell attachment and accelerated...
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