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Tracking nano-metric particles in a biological environment is a very difficult task because of the low signal intensity and the high mobility of these small objects. The task becomes nearly impossible for classical tracking procedures when the targets are labeled with a marker that is not strictly specific, because in this case dynamic structures in the cell are also visible. To address this limitation,...
Cellular endocytosis is a mechanism of great interest in biology, for it regulates the communication between the cell and the external medium. With recent advances in fluorescence microscopy, endocytosis has become a popular candidate for image-based high content screening campains. In this context, we have developed an automated framework comprising robust cell segmentation using coupled shape-constrained...
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