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We report about two-photon activation of a photoactivatable derivative of the Aequorea Victoria green fluorescent protein (paGFP). This special form of the molecule increases its fluorescence intensity when excited by 488 nm after irradiation with high intensity light at 413 nm. The aim in this work was to evaluate the use of two-photon interactions for confining the molecular switching of pa-GFP...
We report about two-photon activation of a photoactivatable derivative of the Aequorea Victoria green fluorescent protein (paGFP). This special form of the molecule increases its fluorescence intensity when excited by 488 nm after irradiation with high intensity light at 413 nm. The aim in this work was to evaluate the use of two-photon interactions for confining the molecular switching of pa-GFP...
Adult-derived stem cells have recently been found to respond in vivo to inductive signals from the microenvironment and to differentiate into a phenotype that is characteristic of cells in that microenvironment. We examined the differentiation potential of an adult liver stem cell line (WBF344) in a cardiac microenvironment in vitro. WBF344 cells were established from a single cloned non-parenchymal...
Adult-derived stem cells have recently been found to respond in vivo to inductive signals from the microenvironment and to differentiate into a phenotype that is characteristic of cells in that microenvironment. We examined the differentiation potential of an adult liver stem cell line (WBF344) in a cardiac microenvironment in vitro. WBF344 cells were established from a single cloned non-parenchymal...
Understanding the motility of subcellular particles like organelles, vesicles, or mRNAs is critical to understand how cells regulate delivery of specific proteins from the site of synthesis to the site of action. The goal of this paper is to present a framework of feature selection, matching, and evaluation for the segmentation and tracking of green fluorescent protein (GFP) labeled subcellular structures...
Understanding the motility of subcellular particles like organelles, vesicles, or mRNAs is critical to understand how cells regulate delivery of specific proteins from the site of synthesis to the site of action. The goal of this paper is to present a framework of feature selection, matching, and evaluation for the segmentation and tracking of green fluorescent protein (GFP) labeled subcellular structures...
A synthetic genetic circuit has been designed whose topology and function echo those of an electronic inverting amplifier. Several variants of this circuit have been built in our laboratory. This paper reports on the testing of one of these variants and contributes to the field both in terms of evaluating the specific amplifier performance and in terms of providing a methodology for performance evaluation...
A synthetic genetic circuit has been designed whose topology and function echo those of an electronic inverting amplifier. Several variants of this circuit have been built in our laboratory. This paper reports on the testing of one of these variants and contributes to the field both in terms of evaluating the specific amplifier performance and in terms of providing a methodology for performance evaluation...
Fusion proteins are an important class of proteins with diverse applications in biotechnology. They consist of 2 or more rigid domains joined by a flexible linker. Understanding the conformational space of fusion proteins conferred by the flexible linkers is important to predicting its behavior. In this paper, we introduce a modeling tool called FPMOD (Fusion Protein MODeller) which samples the conformational...
Fusion proteins are an important class of proteins with diverse applications in biotechnology. They consist of 2 or more rigid domains joined by a flexible linker. Understanding the conformational space of fusion proteins conferred by the flexible linkers is important to predicting its behavior. In this paper, we introduce a modeling tool called FPMOD (Fusion Protein MODeller) which samples the conformational...
Statistical image analysis has emerged as a basic methodology in the study of many real phenomena, which can be represented by sequences of binary images. Recent techniques, such as total internal reflection fluorescent microscopy, allow us to image simultaneously two fluorescent-tagged proteins which are relevant in important cellular processes, such as endocytosis. Here, we model these biological...
Statistical image analysis has emerged as a basic methodology in the study of many real phenomena, which can be represented by sequences of binary images. Recent techniques, such as total internal reflection fluorescent microscopy, allow us to image simultaneously two fluorescent-tagged proteins which are relevant in important cellular processes, such as endocytosis. Here, we model these biological...
We describe a novel automatic approach for vesicle trafficking analysis in 3D+T videomicroscopy. Tracking individually objects in time in 3D+T videomicroscopy is known to be a very tedious job and leads generally to unreliable results. So instead, our method proceeds by first identifying trafficking regions in the 3D volume and next analysing at them the vesicle trafficking. The latter is viewed as...
We describe a novel automatic approach for vesicle trafficking analysis in 3D+T videomicroscopy. Tracking individually objects in time in 3D+T videomicroscopy is known to be a very tedious job and leads generally to unreliable results. So instead, our method proceeds by first identifying trafficking regions in the 3D volume and next analysing at them the vesicle trafficking. The latter is viewed as...
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