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A set of protein pairs predicted to be interacting with high ratio of true positive is valuable for target selection in experiments like protein structure determination. Our goal in this paper is to investigate the problem of finding such a set of protein pairs in an organism by machine learning methods. Yeast genome was taken for this study and support vector machine was adopted as the classification...
A CW type handheld near infrared tissue oximeter called P-Scan tissue imager was developed for real time imaging of tissue oxygen saturation ([StO2]) and hemoglobin concentration ([Hbt]). The probe consists of eight dual-wavelength light sources (690 nm and 830 nm) and eight photon detectors forming a 2.5 cm times 2.5 cm matrix. The local tissue oxygen saturation and hemoglobin concentration was calculated...
This paper describes the detection of vesicular stomatitis virus using a novel capacitive immunosensor technology, whereby the respective antibodies for the antigens were used as a means for chemical detection on separate sensors. Devices were fabricated using standard etching and metal plating techniques, followed by immobilization of antibodies. Detection of antigen was performed by measuring voltage...
To infer protein function by matching local surface patterns, an effective scoring matrix for evaluating surface similarity is critical. In this study, we develop an evolution model of binding surfaces using a continuous time Markov process. We develop a Bayesian Markov chain Monte Carlo method to estimate the substitution rates of amino acid residues with specialized move sets. We then develop scoring...
The identification of protein-protein interactions along with their spatial and temporal localization is vital data for assigning functional information to proteins. Historically, these data sets obtained from fluorescence microscopy, have been analyzed manually, a process that is both time consuming and tedious. The development of an automated system that can measure the location dynamics of the...
Genetic algorithm is used to perform variable selection to determine the ranges of wavelengths in NIR spectral data suitable to be used as predictors in multivariate calibration model via partial least squares. The NIR spectral data consists of three components of active substances, namely human serum albumin (HSA), gamma-globulin and glucose. The wavelength selection is able to improve the calibration...
The investigation of interactions between two kinds of monoclonal antibodies and SARS virus with a label-free protein array technique were presented in this paper. The performance consists of three parts: a surface modification for ligand immobilization/surface, a protein array fabrication with an integrated microfluidic system for patterning, packaging and liquid handling, and a protein array reader...
Polypyrrole (PPy) is one of the important conductive polymers that are widely used in energy storage systems, biosensors and electronics. The electrochemical synthesis of PPy has advantages of simple process, mass production and low cost. In this study, polypyrolle were used to reporterlessly detect DNA and proteins. PPy thin films impregnated with probe molecules were deposited on both microelectrode...
To investigate changes in vascular functions in streptozotocin (STZ)-induced diabetic rats and the effect of interleukin-2 (IL-2), male Sprague-Dawley rats were randomly divided into a normal group, a diabetic control group, and diabetic groups treated with a low dose (5000 U/kg/d) or a high dose of IL-2 (50000 U/kg/d) for five weeks. Rats were injected with STZ (60 mg/kg, i.p.) to induce diabetes...
This paper presents a study on force enhancement and mechanisms of contraction in skeletal muscle. Force enhancement was evaluated in four conceptually different experimental preparations: in vivo human muscles activated through voluntary contractions; isolated, in situ muscle preparations (typically the cat soleus), isolated single fibres (typically from the lumbrical muscles of the frog), and isolated...
Heart failure has been identified as a serious international problem, in particular for aging groups, posing both an increasing number of patients on waiting lists in countries susceptible with Medicare systems and increasing financial burdens. It may be imperative to develop a marker that can identify such problems at an early stage. It is believed that certain proteins have crucial roles in early...
Protein sequence alignments reveal the evolutionary information between homologous sequences. Traditional sequence alignment methods only use sequence information and the structure information from template is ignored. Recently, Kleinjung et al. developed a contact-based sequence alignment method that used the structural information from side-chain contacts. Alignment scores are provided by the CAO...
The influence of low-frequency electromagnetic field irradiating by high-voltage transmission lines on signal transduction of cell in spleen cells of the rates have been studied by molecular-biology techniques. The spleen cells are extracted from skilled rates, which are exposed in the electromagnetic field of high-voltage transmission lines with 4000 V/m and 0.09-0.1 G about 400 days. The quantity...
The accurate estimation of cerebral metabolic rate of O2 (CMRO2) is still an unavailable but highly desired clinical tool. The addition of a further experimental variable to near infra-red spectroscopy (NIRS) allows more information to be gained than has previously been possible. A physiological model is presented here that can be used to estimate CMRO2 from measurements of oxy- and deoxy- haemoglobin...
Nonlinear properties of biological suspensions have been previously presented as a bulk phenomenon, however, some authors consider that they are generated at the electrode-electrolyte interface and are reflected as a bulk property phenomenon. They were mainly ascribed to the H+-ATPase present in the plasma membrane of these cells. In this paper, we describe the construction of a dual-cell nonlinear...
Despite the synthetic peptides inhibit HIV-1 entry; its application of this peptide therapy may be limited due to the high cost of the peptide production and lack of its oral availability. Thus, it is necessary to identify the small molecule inhibitors reacting with the same or overlapping target sites on gp41 recognizing the antiviral peptides. In this work, a small inhibitor (TP1) is docked into...
The reverse engineering paradigm is given increasing attention in computational molecular biology lately. One of the goals is to understand how gene regulatory networks (complex systems of genes, proteins and other molecules) function and interact to carry out specific cell functions. We present an approach for inferring the complex causal relationships among genes from microarray experimental data...
Using the method of directed collagen gel shrinkage, we have been fabricating heart valves and mitral valve chordae. The principle involves mixing solubilized collagen with the appropriate cells. When the collagen-cell mixture is neutralized, soluble collagen reassembles into fibrils and a gel is created. When the gel is mechanically constrained, the collagen fibrils align in the direction of constraint...
To study the effects of 4-hydroxy-2-nonenal (HNE) on cultured human aortic endothelial cells and myocardial cells so as to explore the mechanism of the pathogenesis of atherosclerosis. In situ cell death technique, quantitative DNA damage detection and immunohistochemistry were used to identify the cell apoptosis and DNA damage in cultured human aortic endothelial cells and myocardial cells. Tail...
N-phosphorylamino acids can react to form esters, peptides, phosphoryl ester-exchanged products and phosphoryl group migration products under mild conditions which are very important in researches on the origin of life. It has been proposed that the penta-coordinate phosphorous compounds might be the common intermediates of these reactions, which are the key factor to determine the activities. In...
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