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The authors describe three-dimensional (3D) motion pictures of dynamic micro objects obtained by parallel phase-shifting digital holographic microscope. The microscope consisted of a Mach-Zehnder interferometer, an inverted magnification optical system, a continuous wave laser, and a polarization imaging camera. An alum crystal sinking in aqueous solution of alum was set for a dynamic micro object...
The wave propagation method (WPM) is a method that can accurately simulate inhomogeneous media at high numerical apertures in the micro-optical size range. Recently, the large computing time for 3D-problems could be reduced significantly, thus enabling rapid simulation for micro optical systems.
The rigorous coupled-wave analysis (RCWA) is applied to the simulation of 4π-microscopy. To this end, we combine the concept of structured illumination with coherent two-sided light incidence. It is demonstrated how the latter is integrated into the RCWA framework. With these techniques we simulate two coherent counter-propagating converging beams incident upon a sample as in 4π-microscopy.
Linear polarizers are present in all kind of optical systems. These devices behave as vector projectors in the polarization direction assuming quasi-transverse beams in the paraxial domain. Nevertheless, the performance of polarizers used in combination with focused beams requires a more in-depth analysis. Understanding this behavior is essential in microscopy applications because of the non-negligible...
Many imaging applications rely on sample illumination with known 3-D light distributions. As these 3-D light distributions must satisfy the wave equation, many variables such as the geometry and reflectance of the sample and its environment may cause changes. While in most applications this change is neglected, for many scanning applications where the scanning beam should preserve its characteristics...
In this paper we present an overview of our previously published work on cell identification using digital holography and optical imaging. Various sensing and imaging approaches, and dedicated statistical algorithms are reviewed.
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