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Immunofluorescence is widely used in detection and characterization of circulating tumor cells (CTCs). The process of immunofluorescence staining on cells needs centrifugal force to wash residual fluorescence, which is not only time consuming but also cells harmful. To speed up the staining process, a three-dimensional (3D) microfluidic device is employed in this work. This study also reports the...
Drug delivery system (DDS) is the process of administering a drug-loaded composite to achieve a therapeutic effect in vitro or in vivo. In this paper, we present the fabrication of imiquimod-poly(ε-caprolactone) (PCL) fibre meshes for the melanoma therapy application. The fabrication was based on the electrospinning technique and NIH-3T3 fibroblast cells were cultured on electrospun fibre meshes to...
Biomechanical properties have been revealed as potential biomarkers for distinguishing cancer cells from normal cells. In this work, we report a novel technique using a confining microchannel embedded with microelectrodes for biomechanical phenotyping for floating human cells, including one normal breast cell line (MCF-10A) and two breast cancer cell lines (MCF-7 and MDA-MB-231). The floating cells...
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