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In this paper, a methodology for the analysis of subcellular morphology with chemical specificity, for images obtained by Stimulated Raman Scattering microscopy, is described. In order to detect microstructures inside the cells, a segmentation method based on a threshold algorithm and on a region growing process is proposed. Validation tests on images of polystyrene beads and of on adipocyte cells...
Taking advantage of Stimulated Raman scattering, a label-free single cell imaging technique based on vibrational contrast can be implemented. In this work, the successful realization of a nonlinear microscope, not purchasable in commerce, based on Stimulated Raman scattering is described. In order to test the microscope, polystyrene beads images are carried out. Finally, in order to demonstrate the...
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