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This paper presents a polydimethyl-siloxane (PDMS) microlens bonding on a compact disk (CD) microfluidic device that performs an enzyme-linked immunosorbent assay (ELISA) to enhance the sensitivity of bio-detection. The PDMS microlens can be fabricated with ease using soft replica molding method. A commercial software tool (ZEMAX) has been established to analyze the focal length of the microlens....
This paper presents a new and reliable approach for in-situ imaging and manipulation of membrane protein molecules. The major problematic issue in medicine is the lack of fundamental understanding of the molecular mechanisms that cause major life-threatening and incurable diseases. The development of atomic force microscopy (AFM) opens an entirely new way to address these medical questions. A biocompatible...
Recent development of molecular biological tools has created several research fields such as systems biology, omics studies, and molecular diagnostics. Among them, molecular diagnostics is a new area of clinical diagnosis, and it includes all tests and methods to identify a disease or the predisposition for a disease analyzing DNA, RNA or proteins of an organism. After completion of the human genome...
Bio-molecular recognition is detected by the unique optical properties of self-assembled gold nanoparticles on the unclad portions of an optical fiber whose surfaces are modified with a receptor. To enhance the performance of the sensing platform, the sensing element is integrated with microfluidic chips to reduce sample and reagent volume, to shorten response time and analysis time, as well as to...
We report the use of Atomic Force Microcopy (AFM) in combination with Fluorescence Microscopy (FM) for the investigation of non-Hodgkin's type B cells lymphoma after antibody treatment. Rituximab is used in the treatment this cancer type. It is a chimeric monoclonal antibody directed against the protein Cluster of Differentiation 20 (CD20), which is expressed on mature B cells, including the B cell...
Burkitt's lymphoma (BL) is a highly aggressive malignant tumor with high morbidity rate in children. Patients have different responses to the same therapy due to the molecular heterogeneity. Detailed observation of the surface structure of BL cells to locate the specific receptors (CD20) on the cell surface and measure the binding force between the CD20s and their cognate ligands (Rituximab) may give...
Device consisting of a micro-pattern grating structure with membrane of alternating dielectric materials such as SiO2, Si3N4 and SOG or ITO, is fabricated. Its utilization as a platform for attaching active silane layer such as APTES, further as a sensor after immobilizing of sensing molecules and hybridizing with complimentary molecules is demonstrated. Shifting in peak wavelength is observed when...
The polydiacetylene vesicles-based sensors have been of interest due to their unique chromatic properties, such as blue-to-red color transition. The liposome-like vesicles were immobilized on solid substrates using various polydiacetylene derivatives synthesized with different functional groups, such as, amine, carboxylic acid, and biotin, etc. Through their fluorogenic properties, the label-free...
A novel Biased Reptation model with Electroosmosis (BRE) is proposed for long-chain DNA electrophoresis in microchannels with sub-micron pillar array. The BRE model incorporates effective electroosmosis into the classical BRM model. In addition, the electrophoretic mobilities of different sizes of long-chain DNA, T4 DNA and lambda DNA, at different electric fields were measured. In comparison with...
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