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We investigate the effect of specific antisense oligodeoxynucleotide (ASO DN) inhibition of Fas expression on T cell apoptosis induced by gastric carcinoma cell . Fas receptor (Fas) and Fas ligand (FasL) expressed by the gastric carcinoma cell line SGC-7901 and Jurkat T cells were detected by flow cytometry (FCM) and the ability of Fas L-inducing T cell apoptosis was tested by co-culture assay in...
Propose: Idiopathic orbital inflammatory pseudotumors (IOIP) are inflammatory pseudotumors (IPT) of unknown etiology that develop in the orbit. Due to the lack of well-defined pathogenic mechanisms and diagnostic markers, diagnosis and treatment of this disease remain a significant challenge. Therefore, the aims of this study are to define the etiological factors of IOIP and to identify potential...
We studied the Fas/anti-Fas system to induce killing of Fas-expressing immunocytes with resultant immunosuppression. W7TM-1 tumour cells, a rat T-cell line, were inoculated subcutaneously in BALB/c mice and tumour growth was monitored in untreated mice and in mice treated with RMF2. Prior to treatment with RMF2, we examined the expression of Fas in isolated splenocytes and in tumour infiltrating lymphocytes...
Thinopyrum elongatum can provide a lot of profitable genes for improving the qualities, resistance and agronomical traits of wheat. It is an effective method to transfer the profitable genes of Th. elongatum into wheat by inducing chromosome transloctions. In this study, to inducing wheat-Th. elongatum 7E chromosome translocations, hybrid, backcross and selfcross were done between Chinese spring-Th...
In this issue, the effect of toluene diisocyanate on the mice blood secrum was studied to discuss its mechanism of EOS infiltration and conglomeration, which was the emblematical character of vocational asthma. Effects of TDI on the mass concentrations of IL-4, IL-5 in mice serum and granulocyte-macrophage colony-stimulating factor was observed by double-antibody sandwich enzyme-linked immunosorbent...
This work investigated the potential use of benzoic acid (BA)-based immunoadsorbent, a hydrophobic adsorbent, for autoantibodies selective adsorption therapy. The adsorbent was assessed through chromatographer and batch system assays in terms of adsorption capacity, selectivity for both polycloneantibodies in normal human plasma and pathogenic antibodies in patients' sera. For human immunoglobulin...
Coal mine ventilation air methane can be mitigated by thermal oxidation in a counter-flow reactor. The pressure loss of the gas flowed in honeycomb ceramic which is the key component used in reverse flow reactor was studied in this paper. The mathematical model of single-channel of ceramic honeycomb was established by Fluent software, and the resistance characteristics of the ceramic honeycomb Characteristic...
The activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and concentrations of malondialdehyde (MAD) in serum, spleen and bursa of Fabricius and the concentrations of Immunoglobulin G (IgG) , Immunoglobulin A (IgA), Immunoglobulin M (IgM) and HI antibody titer to Newcastle Disease in serum were detected in chickens with different levels sub-chronic aluminum (Al) intoxication produced...
Rigidity (muscle stiffness) is one of the most disabling symptoms in Parkinson's disease (PD). It is clinically defined as an increased resistance to passive movement of a joint. There is a fundamental gap between mechanistic and applied approaches to understanding this symptom. The objective of the current study was to apply a system identification and modeling approach to differentiating the contributions...
The Electric-Bag Complex Dust Collector (EBCDC) is being widely used for dust removal of flue gas from the coal-fired power station boiler. Electrostatic precipitator (ESP), as a pre-collector, has a certain effect on the post-baghouse. In this paper, systematic experiments are done to study the effect of the filtration resistance, the formation of dust cake and the cleaning process of baghouse unit...
The good seed cells were the key component of cartilage tissue engineering. Because of their own advantages, mesenchymal stem cells (MSCs) became a good choice of seed cells in cartilage tissue engineering field. However, how to regulate MSCs to directly differentiate into chondrocytes was one of the difficulties at present. Sox9 and Cbfa1 were two important transcription factors for chondrogenic...
In this study, artificial antigens for Bensulfuron-methyl (BSM) were prepared by means of two-step procedure of glutaraldehyde method. The hapten BSM was covalently attached to ovalbumin to synthesis immunogen (BSM-OVA), and hapten Benzyl sulfonamide (BS) was linked to BSA to prepare coating antigen (BS-BSA). The conjugates were identified by UV spectrophotometry and SDS-PAGE. After immunized with...
Prediction of B cell epitopes of duck enteritis virus (DEV) VP19c protein encoded by UL38 gene and expression in prokaryotic system were described in the present study. The major antigenic portion of VP19c protein of DEV was predicted and corresponding gene was amplified from the extracted DNA and cloned. The recombinant expression construction(pPAL7-truncated UL38) was identified by the polymerase...
Objective: To construct the recombinant eukaryotic expression vector pcDNA3.1-CEMP1 containing the Human Cementum Protein 1 (hrCEMP1) by PCR and T/A cloning and establish NIH3T3 fibroblast cellline that stably expressing it. Methods: A pair of primers specific for amplifying the DNA fragment encoding hrCEMP1 were designed and synthesized. hrCEMP1 was inserted to the proper sites of vector pcDNA3.1...
Recent studies have demonstrated that the expression of aquaporin1 (AQP1) in endothelia cells played important roles in cell migration, and migration-associated cell function such as wound healing, and neutrophil motility. In the present study, we provided evidence that AQP1 expression in vascular endothelia cells (VECs) during angiogenesis was associated with MRTF-A. Morphology and immunofluorescence...
The aim of this study is to explore the mucosal and systemic antibody immune responses induced by recombinant L.casei expressing the major immunoprotective antigen VP7 of bovine rotavirus (BRV). Two inducible expression vectors containing the antigenic dominant region of VP7 gene, pPG-1-VP7 (cell-surface expression vector) and pPG-2-VP7 (secretory vector), were constructed and electrotransformed into...
In this study, differentially expression genes were isolated from a indica rice cultivar Zhong156 with a race-specific blast resistance gene subjected to the avirulent race ZC15 and the virulent race ZB1 of Magnaporthe grisea at the seedling growth stage. Twenty-five cDNA clones were isolated by using suppression subtractive hybridization combined with reverse Northern blot and confirmed by RT-PCR...
Cyclophosphamide (cy) was used to produce immunosuppressed model mice by intraperitoneal injection. Affusing different-dosage of the female silkworm moth powder, thymus and spleen index, peritoneal macrophage phagocytosis, lymphocyte transformation, serum hemolysin of mice were investigated. The result showed that all the indexes of the tested immunosuppressed mice were improved by the female silkworm...
Maggots are known for their anti-microbial properties in chronic wound healing. This paper presents research investigating the effects of maggot metabolites in combination with ciprofloxacin against Staphylococcus aureus. The screening process used was a microchannel-based separation and mixing system. The results demonstrate that for similar levels of efficacy, the partial substitution with the maggot...
The aim of the study was to develop a procedure of purifying rabbit anti-rat TRβΔ polyclonal antibody from the antiserum. TRβΔ was a newly reported thyroid hormone receptor beta isoform. To gain better understanding of this new receptor, such as its tissue distribution, its specific antibody was needed. In this study, chemically synthesized peptide, which is uniquely existed in the TRβΔ, was used...
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