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China is a great agricultural country. Reasonable development and utilization of rural energy is related to Peasant's human production and life and improvement of rural eco-environment. Because lack of energy is serious in recent years. China strengthened study on biogas technology and got rich findings. But there is relatively backward in extended application of biogas technology. Therefore,this...
The pulA gene enconding a thermostable pullulanase of 844 amino acids was cloned by PCR amplification from the genomic DNA of Thermotoga maritima MSB8. We successfully expressed the pulA gene in a protease-deficient strain Bacillus subtilis WB600, and the expressed pullulanase was secreted into the culture broth. Preliminary results showed that the pullulanase exhibited optimal activity at 90°C, and...
Control-effective flux(CEF) analysis is a useful tool in assessing a metabolism. In this work, a model that utilizes existing knowledge of oxygen and redox sensing/regulatory system to assist elementary flux modes (EFMs), has been developed and CEF analysis was performed to analyze the influence of oxygen level on the transcriptional regulation in Klebsiella pneumoniae. It was found that the efficiency...
Deoiled Jatropha curcas seed meal (DJSM), a chief waste material of oil extraction industry, will be generated on a large scale due to the growing interest in using Jatropha curcas seed oil as a feedstock for the production of biodiesel. Up to now, there is very little work on viable approach for the use of DJSM to the best of our knowledge. This article reports our studies on the utilization of this...
Chinese medicinal insects were added into the media to investigate their effects on the mycelial growth and polysaccharide formation by Ganoderma lucidum SCIM 0819. The experiments were carried out in submerged fermentation using shake flasks. The results show that addition of C. molossus at 5.0 g/L lead to significant increase in intracellular polysaccharides (IPS) concentration from 1.48 ± 0.12...
Baoding city locates on northwestward of the North China Plain, middle section of the Taihang Mountain which is a typical city on groundwater developing in China. Authors summarize the history of groundwater use and contributions of groundwater, analyze environmental geological issues caused by over-exploitation of urban groundwater which are similar with other water-shortage cities. Main environmental...
An efficient in vitro propagation culture protocol was developed for one of the most important herb medicinal plants purple coneflower. Under the protocol, adventitious buds were first regenerated from leaf, petiole and root explants of aseptic seedlings on MS medium with 0.3 mg/l BA and 0.01 mg/l NAA, and then these adventitious buds were rooted and grown into plantlets of certain maturity on MS...
The "metabolism of human society" is a concept used to characterize the processes of energy and material transformation in a society which are necessary for its continued existence. In order to improve the traditional input-output analysis method and the black-box analysis model, the societal metabolic ecosystem of china was divided into different components to establish societal metabolic...
Phosphine (PH3) is a natural gaseous carrier of phosphorus in its biogeochemical cycles, has been found ubiquitously present in the environment. The origin of phosphine in various environmental sites is still under debated, although phosphine evolution used to be reported from microbial habitats. The tentative experiments show that phosphine gas could be produced by aqueous or acid corrosion from...
We studied the reaction to the oxidative component of low temperature stress in tobacco lines, transformed with gene coding for chloroplast ω-3 desaturase with their wild type. The levels of oxidative stress markers malondialdehyde as well as the activity of antioxidative enzymes catalase and peroxidase have been followed at acclimation, 3 and 6 days at 5°C treatment. Low temperature stress resulted...
Microbial Flocculants is a kind of metabolic products with flocculating activity being produced by microbes and secreted outside of cells. It has the characteristics of easy degradability, highly efficiency, non-toxicity and non-secondary pollution. The flocculation mechanism of Microbial Flocculants is more complicated than the conventional flocculation theories. Through summarizing the relevant...
Various phthalates are used extensively as plasticizers in kinds of consumer products. They are harmful for human reproduction and fertility. Mono-(2-ethylhexyl) phthalate (MEHP) is the active metabolite of di-(2-ethylhexyl) phthalate(DEHP),which is most commonly used as a plasticizer and solvent in numerous consumer products. MEHP has been demonstrated to be a reproductive toxicant in rodents, but...
The relationships among chlorophyll fluorescence parameters, chloroplast antioxidant enzyme and active oxygen in the leaves of P. tenuiflora seedlings under Na2CO3 stress was investigated in the article. Results showed that when Na2CO3 stress was less than 0.4%, the activity of antioxidant enzymes (APX, GST, SOD) increased along with the increasing Na2CO3 stress. However, the changes of actual photochemical...
Polyhydroxyalkanoates have attracted much interest as an alternative to traditional plastics since they are biodegradable and biocompatible. In this paper, activated sludge used for PHA production was selected and effects of pH and initial Sludge PHA Content on PHA production were studied. It was found that the aerobic activated sludge obtained the highest PHA content 52.4% under aerobic conditions...
S-system models for biological systems are derived from the generalized mass action law and are typically a group of nonlinear differential equations. Estimation of parameters in these models from experimental measurements is thus a nonlinear problem. In principle, all algorithms for nonlinear optimization can be used to estimate parameters in molecular biological systems, for example, Gauss-Newton...
This project focuses on the improvement of NDV production technology and innovation of the NDV vaccine preparation process with cell culture technology. Methods: To use primary chick embryo cell culture system in preparation of NDV anti-tumor vaccine. Results:In the new vaccine development process, the focused on maintaining and improving NDV titer while reducing the residual bovine serum. So that...
Based on complex ecosystem system, analyze sustainable urbanization from three aspects, development, sustainability and coordination, build three dimension urbanization comprehensive assessment model based on development, sustainability and coordination, appraise urbanization development from 2000 to 2007 in Xiamen by the model, the result indicates, urbanization level from 2000 to 2007 in Xiamen...
With the rapid development of economy part of which is devoted by coal mines, safety evaluation can not be the perfect method to promote the safety level of coal industry especially when factors holding back safe production come up. Thus the early-warning system must be established so as to send out signals before disaster happens. Artificial neural network is the basis in this article to set up early-warning...
Life Cycle Assessment (LCA) is induced to examine the environmental impacts in the life cycle of milk package from polythene, and to compare the environmental impacts from different plastic package waste treatments in this article. The data of the mass and energy fluxes and the environmental emissions are obtained from literatures and sites investigation. The application of LCA by using Eco-Indicator...
The filter media dimensions are the key design parameter in filter system. In this study, the ceramic pellets with diameter of 3-5 mm, 6-9 mm and 10-13 mm were employed in the vermifilters of D1, D2 and D3, respectively, for excess sludge treatment. The aim of this study was to optimize the diameter of filter media based on the stabilization degree of excess sludge and the corresponding physiological...
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