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Adipose tissue is derived from the embryonic mesenchyme and can be isolated easily. The purpose of this study was to investigate the multipotent ability of adipose-derived mesenchymal stem cells and to investigate the molecular characterization of adipose-derived mesenchymal stem cells during chondrogenic differentiation in vitro. Our results showed that adipose-derived mesenchymal stem cells have...
A variety of xenogeneic and allogeneic extracellular matrix (ECM) scaffolds have been promising in various tissue engineering applications, such as the porcine small intestinal submucosa. Yet no particular scaffolds have been optimal for vocal fold regeneration. The decellularized human umbilical vein (HUV) is a novel allogeneic scaffold that has shown some promise for cardiovascular tissue engineering...
CCAAT/enhancer binding proteins (C/EBPs) comprise a family of transcription factors that are critical for normal cellular differentiation and function in a variety of tissues. They are not only involved in normal processes of physiological metabolism but also are related to the occurrence and development of many kinds of disorders. C/EBPs have multiple modes in which they act as activators or inhibitors...
L-ascorbic acid and sodium carboxymethylcellulose were taken as raw material to synthesize polymer material L-ascorbic acid cellulose ester which had antioxidation effect. Antioxidation effect of o benzylgalloyl microcrystalline cellulose ester was appraised in vitro. The results showed that all the different concentrations L-ascorbic acid cellulose ester significantly improved GSH-Px activity of...
β-ketoacyl-ACP synthetase II (KAS II) which catalyzes the elongation of 16:0-ACP to 18:0-ACP, determines the ratio of palmitic acid (16:0) to stearic acid (18:0). Using Rapid Amplification of cDNA Ends (RACE) and a peanut cDNA library we had previously constructed, we isolated a 1867bp cDNA of the KAS II gene containing a 1212bp complete open reading fragment (ORF). Sequence analysis revealed that...
Apoptosis is a form of genetically programmed cell death, which plays a pivotal role in normal development and the maintenance of homeostasis of a variety of tissues including cardiovascular system. Apop-1 is a soluble protein consisting of 194 amino acids with a mitochondrial localization signal at its N-terminus. RT-PCR data revealed that Apop-1 is expressed in all the organs tested. Immunofluorescence...
β-catenin plays an important role in the tissue repair. But its expression and meanings in the diabetic cutaneous wound remains to be determined. In this study, we investigated the expression of β-catenin and PCNA in epidermal stem cells (ESCs) of diabetic rat skin. The diabetes mellitus (DM) rats were induced by a single i.p. injection of streptozotocin. ESCs were extracted from the dorsal skin samples...
Though cartilage tissue is susceptible to damage and difficult to self-repair, cartilage tissue engineering provides an ideal way for the treatment of cartilage defects. Mechanical environments have significant effects on the growth and development of cartilage. The research of mechanical states of repaired articular cartilage by tissue engineering has significantly clinical value. According to the...
Use fresh Phaseolus vulgaris as raw materials, extracting Phaseolus vulgaris saponin by ultrasonic-ethanol method, determining the saponin content by vanillin- perchloric acid colorimetric method, the Saponin content in crude extract powder is 3.149%. Anatomised the stomach and intestine of effected mice after acute toxicity test of oral gavage mouse by Karber method, and observed epithelial tissue...
The activities of superoxide dismutase (SOD) and concentrations of malondialdehyde (MAD) in cerebrum and cerebellum and pathomorphology of brain tissue were detected in chickens with different levels sub-chronic aluminum (Al) intoxication produced by continuous peritoneal injection of fixed volume and different gradient aluminum trichloride (AlCl3). The results showed that the activities of SOD in...
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