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The explants (root tip, shoot tip, stem segments and leaf segments) from Phalaenopsis hybrid were used in this experiment. The effects of NAA, 6-BA, AC, CM and above explants on callus induction on KC medium were investigated by using orthogonal design (L16(54)). The results indicated that the optimum medium for callus induction is 6-BA 4.0 mg/L+ NAA 0.4 mg/+CM 100 ml/L+AC 0.4 mg/L, and stem segment...
β-ketoacyl-ACP synthetase II (KAS II) which catalyzes the elongation of 16:0-ACP to 18:0-ACP, determines the ratio of palmitic acid (16:0) to stearic acid (18:0). Using Rapid Amplification of cDNA Ends (RACE) and a peanut cDNA library we had previously constructed, we isolated a 1867bp cDNA of the KAS II gene containing a 1212bp complete open reading fragment (ORF). Sequence analysis revealed that...
Kosteletzkya virginica is a halophyte species which could be used for production of industrial raw material such as biodiesel and for developing ecologically sound saline agriculture as well as for tideland soil improving. Using Rapid Amplification of cDNA Ends (RACE) method, a 1610 bp cDNA of stearoyl-ACP desaturase (SAD) gene containing a 1188 bp complete open reading fragment (ORF) was firstly...
Heavy metal accumulations in edible plants are commonly reported by scientist to alert the public on potential risks of toxicity effects. The study was conducted to determine the heavy metal accumulation in different part of the plant tissue namely Ipomoea reptans and Helianthus annuus. Spent batteries were utilized as the source of contamination with natural leaching process. Results indicated that...
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