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We have developed a hybrid optical tomography system combing optical coherence tomography (OCT) and line-scanning fluorescence laminar optical tomography (FLOT). This system could be used for concurrent depth-resolved tissue-structural and molecular imaging.
We demonstrate the advantage of combining high-resolution Fourier domain optical coherence tomography (OCT) with wide-field time-gated fluorescence lifetime imaging microscopy (FLIM) for a comprehensive morphological and biochemical characterization of atherosclerotic vulnerable plaques (VP).
Fluorescence imaging with resolution ten times better than the diffraction limit in three dimensions over a depth of field of 2 mum is demonstrated with a widefield microscope that exhibits a double-helix point spread function.
An endomicroscope with enhanced signal collection efficiency was developed using customized double-clad fiber and aspherical compound-lens. Ex vivo two-photon fluorescence imaging of epithelial tissues was demonstrated for the first time with an all-fiber-optic scanning endomicroscope.
We present a monolithically integrated near-infrared fluorescence sensor incorporating a dielectric emission filter for in vivo applications. We successfully integrated a dielectric emission filter (OD3) onto a low-noise detector and sensed 50 nM fluorescent dye concentration.
We demonstrate parallel three-dimensional (3D) tracking of multiple fluorescent microspheres with nanometer scale accuracies by engineering the 3D point spread function of a wide-field microscope to present a double-helix along the optical axis.
We propose and develop a nanoprobe-based technique for characterizing femtosecond laser pulses. A preliminary demonstration based on the measurement of the interferometric autocorrelation trace through two-photon fluorescence from a nonlinear nanoprobe is reported.
Three-photon fluorescence and lasing from ZnSe was observed for a kilojoule-class, 100 picosecond pulse, Nd:glass laser excitation. In this work, the emission properties and its excitation energy dependence were investigated for low and high-energy excitation.
We present experiments and simulations that show the microscopic fluorescence resonance energy transfer (FRET) donor-acceptor distance can be determined using a diffusion model. The approach could lead to deep tissue in-vivo FRET imaging.
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