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We demonstrate three-dimensional imaging by wide field optical coherence tomography with high-speed CMOS camera. A sample volume of 2.3times2.3times1.1mm3 (corresponding to 256times256times300 pixels) was imaged at 10 volumes/s by the scanning reference mirror.
A synchronously pumped, fs-OPO with automated tuning in combination with an optimized scanner / microscope / detection system is used for MPE- and SHG microscopy on biological samples. The system and first results are presented.
We investigated the excited-state ultra-fast dynamics of genetic fusions that link a Green Fluorescent Protein to an antibody fragment. Spectrally resolved pump-probe spectroscopy allowed us to discriminate between biolocally-active soluble and inactive insoluble antibody fusions.
We use a single supercontinuum laser source to simultaneously excite multiple types of fluorescent dyes and collect the entire emission spectrum ranging from visible to near infrared, without using band pass filters and dichroic mirrors.
We demonstrate that microbubbles generated through optical breakdown in tissue phantoms or individual cells are controllable with laser parameters. The controlled microbubbles can be used as an acoustically detectable agent for diagnostic and therapeutic applications.
Fourier domain mode locking is a new operation regime of lasers. Highly chirped frequency swept waveforms rather than short pulses are generated. The mechanism and its application for ultrahigh-speed biomedical OCT imaging are discussed.
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