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Our research group has purified an antioxidant selenium-containing protein (Se-GL-P) from Se-enriched Ganoderma Lucidum (Se-GL), its characteristics has been determined partly. In this study, the characteristics of Se-GL-P were identified further, in which the contents of selenocysteine (Se-Cys) and selenomethionine (Se-Met), isoelectric point (pi), and peptide mass fingerprint (PMF) were involved...
Signal peptides are short regions of amino acid residues, which have become a crucial tool in finding new drugs or reprogramming cells for gene therapy. Owing to the rapidly increasing number of protein, it is highly demanded to develop the automated algorithm to identify the signal peptides. Recently, we had adopted a new alignment kernel function to identify secretory proteins. Compared with previous...
The effect and mechanism of gastric juice secretion by electroacupuncture at "Yanglingquan" point with a variety of modern electronic apparatuses were studied.Before and after Cimetidine Injection injected, the "Yanglingquan" points on rabbits were respectively stimulated with electroacupunctures to observe the flux of gastric juice secretion in the experiment. The result indicates...
Semustine (Me-CCNU, 1-(2-chloroethyl)-3-(4-trans- methylcyclohexyl)-l-nitrosourea) is significant anticancer agents in the clinical treatment of human malignancies. Several lines of evidences indicate that its cytotoxic activity is related to the interstrand cross-linking of DNA. In this study, the agarose gel electrophoresis and fluorometric assay were carried out to detect the NDA interstrand cross-linking...
To explore how to calculate the effect of solanine on the Michaelis constant and the maximum reaction rate of NAT, high performance liquid chromatography (HPLC) was used, with 2-AF as substrate, and the rate at which 2-AF is acetylated into 2-AAF in intact HepG2 cells or in the cytoplasm of HepG2 cells as the reaction rate. The double reciprocal plot was made, with 1/S (reciprocal of the concentration...
G-protein-coupled receptors (GPCRs), the largest family of cell surface receptors play an important role in production of therapeutic drugs. However, the functions of many of GPCRs are unknown. Hence we develop an new method for classifying the family of GPCRs. It is difficult to predict the classification of GPCRs by means of conventional sequence alignment approaches because of their highly divergent...
Streptococcus pneumoniae is the major causative organisms of meningitis, bacteremia, and pneumonia. The drug resistance of S.pneumoniae to antibiotics showed ascending tendency. The present study cloned the full-length HMG-CoA reductase (HMGR) gene of S.pneumoniae and expressed in E.coli BL21 (DE3). The His-tag containing recombinant protein was purified by Ni-NTA affinity chromatography. Statins...
The prediction of the binding affinity of protein- ligand complexes is important for the molecular docking and rational drug discovery. In this study, we have analyzed the descriptors, which affect the binding affinities of protein-ligand complexes, from five dimensions, including protein-ligand interactions, protein properties, structural and physicochemical descriptors of ligands, metal-ligand bonding,...
Cytochrome P450 2C9 (CYP2C9) is among most important members of the cytochrome P450 enzyme superfamily, which metabolizes many important exogenous and endogenous compounds in many species of microorganisms, plants and animals. CYP2C9 is related to the oxidative of 16% of all therapeutics in clinical use and has adverse drug effects, for example, enzyme induction and inhibition. In order to understand...
New target controlled infusion (TCI) for propofol using a hybrid physiologically based pharmacokinetic (PBPK) model was proposed in this paper. The hybrid model was composed of a three-compartment mamillary systemic model and a two-compartment brain model. A closed-loop control algorithm with brain concentration as the control variable was used to calculate the infusion. Finally, the dose regimen...
While relational databases have become critically important in business applications and web services, they have played a relatively minor role in scientific computing, especially in computer-assistant drug discovery, which has generally been concerned with modeling and simulation activities. However, massively parallel database architectures are beginning to offer the ability to quickly search through...
This paper utilizes the BP neural network to establish a prediction model of cyclosporine A blood concentration by analyzing the factors which influence the blood concentration. To avoid its defect of easily getting into the local extremum, we present a prediction model that is based on the genetic algorithm and BP network (GA-BP) by using the genetic algorithm with a good global searching ability...
To determine the effect of osthole on protein expression of PPARalpha/gamma and related target molecules such as CYP7A and DGAT in the liver of hyperlipidemic fatty liver (HFL) rats, and to investigate the possible mechanism of treating HFL. HFL rat model was established by feeding the Sprague-Dawley rats with fat milk for 6 weeks. The experimental rats were then treated with 5-20 mg/kg osthole for...
Acyclovir-loaded PAN hollow fibers were prepared with dry-wet spinning processes. The co- blendine method was employed to prepare the spinning liquid. The prepared hollow fibers had a diameter 01 300 mum. The drug content in the hollow fibers is lower than that in the spinning liquid, 20.28% of the drug was lost during the spinning processes. SIn vitro dissolution tests indicated the obvious initial...
Fine and continuous ibuprofen-loaded PAN/PCL fibers were successfully prepared using wet spinning processes. The co-dissolving method was employed to prepare the spinning solution. The prepared filaments had a diameter of 131 plusmn 24 mum. The drug content in the fibers was found to be a little lower than that in the spinning liquid, 10.7% of the drug was lost during the spinning processes. Polarized...
The extractives from masson pine (Pinus massoniana L.) bark has been used in medicine successfully. Therefore, Py-GC/MS was used to analyze the high-grade resource recovery of benzene/ethanol extractives of masson pine wood. Relative content of each component was determined by area normalization. The main and abundant constituents of 590deg pyrolysis product of masson pine wood werel-phenanthrene...
As the noble gardening tree growing specifically in China, Osmanthus fragrans is widely used to produce high-grade essence, special drugs and aromatic tea form its flowers. However, O. fragrans leaves are still underutilized and hence wasted. Therefore, in order to better utilize O. fragrans leaves and hence to expand its utility values and industrial scopes, we attempted to analyze the bioactive...
The binding of efonidipine to bovine serum albumin (BSA) was investigated by fluorescence spectroscopy and circular dichroism (CD) technique. The quenching mechanism of fluorescence BSA by efonidipine showed that efonidipine quenched the fluorescence of tryptophan residue mainly through the collision mode. The thermodynamic parameters DeltaH0 and DeltaS0 were calculated to be 75.95 kJldrmo-1 and 342...
A novel fast dissolving drug delivery device (DDD) with loose powders in it was designed and fabricated using the three dimensional printing (3DP) process. Based on computer-aided design models, the DDD containing the drug acetaminophen were prepared automatically by 3DP system. The inner powder regions were carried out by depositing the binder solutions onto selected regions during the layer-printing...
To analyze the association between the 5- HT2A receptor gene polymorphism and persistent somatoform pain disorder in Chinese, and to investigate the relationship between the analgesic efficacy of fluoxetine and 5-HT2A receptor gene polymorphism. 51 patients with persistent somatoform pain disorder were administered with fluoxetine(20 mg/day) for 6 weeks. The response to fluoxetine was assessed by...
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