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The new gel-immobilization method for preparing a single stranded DNA (ssDNA) template has been described for single nucleotide polymorphisms (SNPs) genotyping by bioluminometric assay based on allele-specfic extension reaction. In this method, PCR products amplified by a primer modified with acrylamide group at the 5'-terminal is copolymerized with acrylamide monomer to form gel. After the ssDNA...
A micro-pillar chip is proposed and developed to separate the amino acids, phenylalanine and tryptophan, through the aqueous two-phase system (ATPS). The surface properties of micro-pillars, specifically hydrophobicity and hydrophilicity for separation are also investigated. Because their isoelectric points (pI values) are similar, these amino acids are difficult to separate by general extraction...
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