Walking for health is currently popular, it should be discussed the effects of insole types. We adopted insoles composed of freely moving micro-beads, expecting the enlargement of sensitivity for muscle fatigue and balance. The target muscles and the locations of surface electrode were determined with the muscle synergies and the spatiotemporal distribution pattern measured with a matrix surface electrode. From 15 healthy mail participants the time courses of %iEMG were acquired for total 100 strokes walk on the treadmill with the speed of 3 km/h. The results showed that induced balance related effects were observed with freely moving micro-beads less filled insole compared with a barefoot: %iEMG decreased at thigh, gluteus, and erector spinae muscles, and significantly at lower leg muscles. With a lot of filling quantity, %iEMG increased at gluteus, erector spinae, and lower leg muscles, while there were not significantly increased at thigh muscles. These might be link to the compensation for the balance stability as stochastic resonance effects.