This paper deals with robust control of Electro-Hydraulic Load Simulator(EHLS). An electro-hydraulic system consists of uncertainties such as parametric uncertainty due to hydraulic properties of working fluid varying with temperature, nonlinear relation between flow rate, load pressure, and input of a servo valve and disturbance acting on actuators. Therefore, these uncertainties and disturbance called perturbation should be estimated. For this aim, the Sliding Mode Control with Perturbation Estimation(SMCPE) is applied to EHLS in this study. The SMCPE is to estimate the perturbation based on concept of Time Delay Control(TDC). Thus, performances of both tracking and estimation are studied with respect to various sampling time and the tracking control is simulated in time domain under various disturbance conditions. It is concluded that the application of SMCPE can be an effective control scheme for EHLS.