Given the limited spatiotemporal resolution of 3D echocardiography, simultaneous assessment of all ventricular myocardial segments can clinically be performed using multi-plane acquisitions (MP) — i.e. biplane (BP) or triplane (TP). However, the wider field of view of MP impairs spatiotemporal resolution, thus hindering the performance of e.g. speckle tracking. Multi Line Transmit (MLT) beam forming (i.e. simultaneously transmitting multiple focused beams) has been shown an appealing approach to improve temporal resolution without sacrificing image quality. At IUS 2016, an MLT sequence dedicated for MP imaging was presented and evaluated using computer simulations. The aim of the present study was to implement and validate this sequence on an experimental ultrasound system.