High-frequency ultrasound (>20 MHz) imaging has gained widespread attention due to its high spatial resolution being useful for basic cardiovascular and cancer research involving small animals. The sampling rate of the analog-to-digital converter in a high-frequency ultrasound system usually needs to be higher than 120-MHz to satisfy the Nyquist requirement. However, the sampling rate is typically within the range of 40–60 MHz in a traditional ultrasound system, which is not compatible with high-frequency ultrasound imaging. We propose a delayed-excitation method in this study for performing high-frequency ultrasound imaging with a traditional data acquisition scheme.