We report a flexible self-generated triboelectric microfluidic sensor based on liquid-solid triboelectrification and electrostatic induction mechanism. The flexible microfluidic sensor can be conformally attached on curved surfaces and work under two sensing modes - triboelectric single electrode mode and interdigital (IDT) electrode mode. The microfluidic sensor has linear force sensing range up to 40 N with sensitivity of ∼0.03 N−1. It is capable of simultaneously monitoring both the magnitude and frequency of the pressure/force. Due to the multiple underlying electrodes design, the microfluidic sensor can be integrated with microfluidic system as a self-generated flow rate sensor. The proposed microfluidic sensor shows great potential for diversified applications in pressure/force sensing, flow rate sensing and human motion monitoring.