Fiber optics is the most promising technology for distributed temperature sensing. The paper investigates the characterization of probes based on single or multiplexed fiber Bragg gratings, specifically conceived to evaluate the temperature distribution in applications that imply large temperature gradients, such as in laser induced thermal treatments of solid tumors. A setup for the characterization of fiber Bragg grating sensors in non uniform temperature conditions is described and examples of applications in case that mimic actual working conditions are reported.