Bioenergy and biomass production from paddy microbial fuel cell (PMFC) was assessed throughout the life cycle under greenhouse condition. Performance of traditional sediment PMFC was compared to the earthen membraned double chamber PMFC (DPMFC). Average power density normalized to a plant growth area (PGA) was 87% higher in DPMFC than SPMFC with same electrode material and area, while polarization curve depicted the maximum power of 60 mW/m2 (anode area) in DPMFC, which is greater by 55 % in SPMFC. Maximum bioenergy harvest was achieved at vegetative phase, which was declined with the progression of life cycle. Tillering phase outperformed other growth stages accompanied with maximum greenness with an efficient exudation of rhizodeposition. Cessation of catholyte significantly reduced current density in DPMFC. Plant growth and biomass production was promising under greenhouse conditions. Near the end of life cycle, soil cathode performed better than water cathode. Therefore, PMFC offers dual advantage of biomass and bioelectricity under greenhouse ensuring it as a prominent green energy.