This paper presents an experimental investigation of excised human skin tissue material parameters by THz Time Domain Spectroscopy in the band 0.1–2.5 THz. The results are used to evaluate the channel path loss Nano-electromagnetic communication. Refractive index and absorption coefficient values are evaluated for dermis layer of the human skin. Results obtained illustrate the effect of hydrated tissue on channel parameters and provide the optimum distance, which can be utilized for effective communication inside the human skin.