This paper describes the window shaped fractal antenna and uses it as application in RFID by reducing the size of an antenna along the use of fractals technique. The proposed antenna possess the properties of miniaturizations by 15.1% in second iteration of window shaped based antenna. The FR4_epoxy uses as the substrate with height of 1.6mm. The proposed antenna has been fabricated and tested on analyzer, the outcome result comparison with simulated result, have found the similar characteristics. The return Loss (S11) < −10dB of the proposed antenna at second iteration found frequencies of 2.25 GHz, 3.5GHz and 5.3GHz.